Manufacturers purification process impact

Manufacturing approaches for selected bioproducts of the new biotechnology impact product recovery and purification. The most prevalent bioseparations method is chromatography (qv). Thus the practical tools used to initiate scaleup of process Hquid chromatographic separations starting from a minimum amount of laboratory data are given. 

Tlie development of validated manufacturing processes is a prerequisite for pharmaceutical application of the newer biotechnologicals, such as DNA for plasmid-based genes in vaccines and gene therapy. Using bioprocess-design information it is possible to create efficient and consistent processes for these materials. Key issues are the required piu ity, the sensitivity of the chromosomal DNA and larger plasmids to hydrodynamic forces, and the impact of the various characteristics of plasmids on the recovery and purification of DNA for pharmaceutical purposes. " " ... 

The alkylation of toluene with methanol has been investigated for many years as a potential alternative route to / - Qflene, ethylbenzene, and styrene. Conventional / -xylene production from petroleum reformate requires costly purification and separation from jQ lene isomers and other aromatics. A process that selectively produces /)-xylene could have a significant commercial impact by eliminating the need for p-xylene separation. Furthermore, styrene or ethylbenzene production from methanol and toluene is desired as part of the development of processes based on Cl feedstocks rather than ethylene or propylene feedstocks [48], Para- xyl ae is used primarily in terephthalic acid production, a major component of polyester manufacture. 

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