Bioprocess design

Dr Alexei Lapkin is a Senior Lecturer in Chemical Engineering at the University of Bath, UK, where he is also a Deputy Director of the Centre for Sustainable Chemical Technologies. He obtained his Master in Chemistry from the Novosibirsk State University, Russia, and Ph.D. in Chemical Engineering from the University of Bath. His research is focused on process intensification, bioprocessing, design and applications of functional materials. 

Process economics for biological products was discussed by Harrison et al. (op. cit., pp. 334—369) and Datar and Rosen [in Asenjo (ed.). Separation Processes in Biotechnology, DeUcer, New York, 1990, pp. 741-793] at length, and also by Ladisch (op. cit., pp. 401—430). They provided some illustrative examples with cost an yses. Bioprocess design software can also prove helpful in the overall design process (Harrison et al., op. cit., pp. 343-369). 

Decolorization of azo dyes by WRF technology improvements will require integration of all major areas of industrial biotechnology novel enzymes and microorganisms, functional genomics, protein engineering, biomaterial development, bioprocess design and applications. 

Petrides D (2000), Bioprocess Design, Bioseparation Textbook (Intelligent Inc., Super-pro Designer), available at http //www.intelligen.com (accessed November 2006). 

Weuster-Botz, D., Development, parallelization, and automation of a gas-indudng milliliter-scale bioreactor for high-throughput bioprocess design (HTBD), Biotechnol. Bioeng., 89(5), 2005, 512-523. 

Tlie development of validated manufacturing processes is a prerequisite for pharmaceutical application of the newer biotechnologicals, such as DNA for plasmid-based genes in vaccines and gene therapy. Using bioprocess-design information it is possible to create efficient and consistent processes for these materials. Key issues are the required piu ity, the sensitivity of the chromosomal DNA and larger plasmids to hydrodynamic forces, and the impact of the various characteristics of plasmids on the recovery and purification of DNA for pharmaceutical purposes. " " ... 

KALIL, S. J. MAUGERY, F. and RODRIGUES, M. I. Response surface analysis and simulation as a tool for bioprocess design and optimization. Process Biochem. 35, 2000, pp. 539-550. 

Figure 2.14. Strategies in bioprocess design based on the macroscopic principle and using the formal kinetic concept as part of an integrating strategy. The strategy incorporates the spatial change of mass flux through area Vnl (kg/m h) and the rate of consumption or formation (kg/m h). rds, rate-determining step qss, quasi-steady-state. (From Moser, 1983b.)...

Stoichiometry (Balancing Methods) Applied in Bioprocess Design... 

Zhou, Y.H., Titchener-Hooker, N.J. (1999) Visualizing integrated bioprocess designs through "windows of operation . Biotechnol Bioeng., 65 (5) 550-557. 

The clinical potential of umbilical cord blood-derived stem and progenitor cells has been demonstrated, but the development and success of these therapies is limited by the need for increased cell numbers. Ex vivo expansion has been widely studied as a method to overcome this limitation. To be effective, cell therapy bioprocess design and optimization needs to incorporate a few basic criteria (Kirouac and Zandstra 2008) (i) assessment of relevant cell properties (ii) measurement and control of key parameters (hi) robust predictive strategies for interrogating and evaluating the many parameters that may... 

Schmolzer, K., Madje, K., Nidetzky, B., and Kratzer, R. (2012) Bioprocess design guided by in-situ substrate supply and product removal process intensification for synthesis of (S)-l-(2-chlorophenyl) ethanol. Bioresour. Technol., 108, 216-223,... 

Bioprocess Design How to design a process to manufacture 1000 pieces of live tissue per week Nobody has come up with a well-thought-out design methodology yet. The process must be scalable, economical, and more impoitantly, as well as good manufacture practice (GMP) compliant. 

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