Mannosyl transferases

CDG-Id Dol-P-Man Man5GlcNAc2-PP-dolichol mannosyl-transferase hALGi 3q27 601110... 

CDG-Ig Dol-P-Man Man7GlcNAc2-PP-dolichol mannosyl-transferase hALG12 22ql3 607143... 

CDG-Ii GDP-Man ManiGlcNAc2-PP-dolichol mannosyl-transferase hALG2 9q22 607906... 

Lowary et al. synthesized oligomannoside derivatives to find out the optimal length required by (l 6)-mannosyl transferase for LAM biosynthesis [267, 268] and the specificity of a(l - 4)-mannosy I transferase involved in mycobacterial methylmannose polysaccharide biosynthesis [269], Oligoarabinofuranosides containing C3-modified Ara/residues were also synthesized as the inhibitor of arabinofuranosyltransferases from mycobacteria [270],... 

Kido, N., Torgov, V.I., Sugiyama, T., Uchiya, K., Sugihara, H., Komatsu, T., Kato, N., Jann, K. Expression of the 09 polysaccharide of Escherichia coli sequencing of the E. coli 09 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system. J Bacteriol 177 (1995) 2178-2187. 

Buurman ET, Westwater C, Hube B, Brown AJ, Odds FC, Gow NA Molecular analysis of CaMntlp, a mannosyl transferase important for adhesion and virulence of Candida albicans. Proc Natl Acad Sci USA 1998 95 7670-7675. 

Several glycosyltransferase genes in the gene clusters of S. enterica 0-antigen (serogroup B, C2, El) biosynthesis have been reported. In theses studies mannosyl- and rhamnosyl transferase genes were identified and characterized [142]. Mannosyl transferases and rhamnosyl transferases form two separate families, but there was no relation between them nor were any conserved motifs. 

Reaction M is catalysed by GDP-mannose dehydrogenase [EC 1.1.1.132] for which the co-factor is two moles of NAD+ and reaction N by GDP-mannuronic acid mannuronan mannosyl transferase [EC 2.4.1.33]. ManA residues in mannuronan are convertible to L-GulA residues by a C-5 epimerase for which the substrate is polymeric [243, 244, 253]. At high concentrations of Ca2+ a polymer with a high L-GulA content is produced. There is evidence of a multiple attack mechanism. The epimerase is located extra-cellularly and it does not react with ManA residues in the polymer that are acetylated. In a cell free system of the brown seaweed... 

Different maimosyltransferases transfer mannose and 4-deoxymannose from their respective GDP adducts to acceptors. al,2-Mannosyltransferase was employed to transfer mannose to the 2-position of various derivatized a-mannosides and a-mannosyl peptides to produce the Manal,2Man structural unit [29,30]. A recent report indicates that mannosyl-transferases from pig liver accept GlcNAcpi,4GlcNAc phytanyl pyrophosphate, an analogue in which the dolichol chain of the natural substrate is replaced by the phytanyl moiety [31]. 

Membranes with a density of about 1.1 g/cc were used as the source of the crude mannosyl-transferase (12). Mn was required... 

Figure 4. Distribution of C-mannose-containing saccharides on paper chromatogram in a system containing GDP-)mannose, peptidophosphogalacto-mannan and components of mannosyl-transferase system. The reaction mixture ( 2) was incubated at 25°C for 1 hr and the reaction stopped by heating for 2 min at 100°C and the reaction mixture was extracted with CHClf methanol, 2 1 (v/v) and the organic and aqueous layers chromatographed (12). The chromatograms were removed from the tank, driedy and cut into...

Figure 8. Location of C-mannosyl residues derived from C-phosphogalacto-mannan following acetolysis (13). A reaction mixture containing 30 mg phospho-galactomannan, 125 fig of crude mannosyl-transferase, and other components of the mannosyl-transferase system (13) were incubated for 2 hr and the reaction stopped and phosphogalactomannan reisolated. Twenty mg of phosphogalactomannan was obtained. The polymer was subjected to acetolysis and the products were deacetyl-ated and fractionated on Bio-Gel P-2 as...

Each enzyme is specific for the sugar transferred, its nucleotide precursor, the residue to which it is transferred and the bond formed. All are effectively irreversible transfers and all the enzymes are membrane-bound. The rhamnosyl and mannosyl transferases can be solubilised with non-ionic... 

Activation of Mannosyl-transferase II by Specific Phospholipids , Journal of Biological Chemistry, 257, 9025-9... 

Lehle, L. Tanner, W. (1974), Membrane-bound Mannosyl Transferase in Yeast Glycoprotein Biosynthesis , Biochimica et Biophysica Acta, 350, 225-35 Lehle, L. Tanner, W. (1975) Formation of Lipid-bound Oligosaccharides in Yeast , Biochimica et Biophysica Acta, 399, 364-74... 

Orchard MG, Neuss JC, Galley CM, Carr A, Porter DW, Smith P, Scopes DI, Haydon D, Vousden K, Stubberfield CR, Young K, Page M. Rhodanine-3-acetic acid derivatives as inhibitors of fungal protein mannosyl transferase 1 (PMTl). Bioorg. Med. Chem. Lett. 2004,14, 3975-3978. 

Mammary gland tumor, 991 Management, 543 Mandelin, 535 Manduca sexta, 4096 Mangifera indica, 1553 Mangiferin, 1553 Mannich cyclisation, 50 Mannosyl transferases (MTs), 1270 Manzamine-type, 1293 MAO. See Monoamine oxidase (MAO) MAPKs. See Mitogen-activated protein kinases (MAPKs)... 

Korner C, Knauer R, Stephani U, et al. Carbohydrate deficient glycoprotein syndrome type IV deficiency of dolichyl-P-Man Man5GlcNAc2-PP-dolichyl mannosyl-transferase. EMBO J 1999 18 6816-6822. 

Considerable attention continues to be given to disaccharides containing amino sugars. Compound 69, which comprises chitobiose linked to dolichol via an a-1-pyrophosphate bridge, has been prepared chemically as a substrate for enzymic glycosylation of peptides, and the chitobiose component was found to be transferable to a peptide.In related work, compounds 70 and 71 were prepared starting from chitobiose octacetate, and the former acts as an efficient acceptor for mannosyl transferases. The anhydrochitobiose... 

The simple analogues (36) of the natural mannosyl transferase acceptor substrate (GlcNAc)2 pyrophosphate-dolichyl have been synthesized from chitobiose octaacetate (35) as shown in Scheme 6. ... 

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