Maltodextrin binding protein

Betton, J.-M., Jacob, J. P., Hofnung, M., and Broome-Smith, J. K. (1997). Creating a bifunctional protein by insertion of /3-lactamase into the maltodextrin-binding protein. Nat. Biotechnology, 15, 1276-1279. 

Evenas, J., Tugarinov, V., Skrynnikov, N. R., Goto, N. K., Muhandiram, R., and Kay, L. E. (2001). Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy./. Mol. Biol. 309, 961-974. 

Sharff, A. J., Rodseth, L. E., and Quiocho, F. A. (1993). Refined 1.8-A structure reveals the mode of binding of beta-cyclodextrin to the maltodextrin binding protein. Biochem. 32, 10553—10559. 

A rather widespread family of proteins, found in the periplasmic space of gramnegative bacteria, complexes certain small molecules and allows them to be transported through the cell wall or activate chemotaxis. Each of these functions involves a consecutive interaction with specific membrane proteins. The molecules transported are amino acids, sulfate, mono- and oligosaccchrides. In this way ABP complexes L-arabinose (K 0.98 x 10 M), and MBP (maltodextrin-binding protein) complexes maltose (ATj 35 x 10 M) and maltodextrins. It is in this series that are found the strongest possible bonds between sugars and proteins. The dissociation rate ( i 1.5 s ) is indicative of the upper limit of the ionic transport rate. Hydrogen bonds... 

Spurhno, J.G., Lu, G.Y. and Quiocho, E.A. (1991). The 2.3-A resolution structure of the maltose-binding or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis. ]. Biol. Chem. 266, 5202--5219. 

Duan, X., Hall, J. A., Nikaido, H., and Quiocho, F. A. (2001). Crystal structures of the maltodextrin/maltose-binding protein complexed with reduced oligosaccharides Flexibility of tertiary structure and ligand binding./. Mol. Biol. 306, 1115-1126. 

A review of enantiomeric separations by CE using polysaccharides as chiral selectors has been reported (217). Ionic and neutral polysaccharides (e.g., heparin, chondroitin sulfate, dextrin, and maltodextrins) have been used to resolved enantiomers. Racemic acidic drugs were resolved with maltodextrins (218), while heparins and cyclodextrins were used to resolve oxamigue (219). A large number of drugs have been found to bind enantioselectively to proteins. 

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