Matrix-assisted laser desorption/ionization time-of-flight MALDI-TOF

MALDI-TOF matrix-assisted laser desorption ionization time of flight... 

The use of diisoprop anolamine in the synthesis of the hyperbranched poly-esteramide resins has led to defined molecules according to the predicted structures (Fig. 7) as confirmed by MALDI-TOF (Matrix Assisted Laser Desorption Ionization - Time Of Flight) and ESI (Electro Spray Ionization) mass spectra... 

MALDI-ToF Matrix-assisted laser desorption ionization - time of flight (detection) ... 

MALDI-TOF Matrix-assisted laser desorption ionization time-of-flight MAP Mitogen-activated protein, nsnally referring to a protein kinase such as M AP-... 

The methods for each study are divided into the initial protein separation step, a second separation step if applicable, the type of mass analysis, and the software used for peptide identification. ID = one dimensional polyacrylamide gel electrophoresis, 2D = two dimensional polyacrylamide gel electrophoresis, MS = mass spectrometry (peptide mass fingerprinting), MS/MS = tandem mass spectrometry, MALDI-TOF = matrix assisted laser desorption/ionization-time of flight, MS FIT = software from Protein Prospector, http //prospector.ucsf edu/, ESI = electrospray ionization, Q-TOF = quadrupole-time of flight, PPSS2 =Protana s Proteomic Software Suite (ProtanaEngineering, Odense, Denmark), Mascot = Matiix Science, http //www.matrixscience.com/, TOF-TOF = MALDI plus TOF tandem mass spectrometry, RP-HPLC = reverse phase high performance liquid chromatography, Q-IT = quadrupole ion trap, LIT = linear ion trap. Bioworks = Thermo Electron Corporation. 

Abbreviations HPLC, high perfonnance liquid chromatography LSIMS, liquid secoiulary ion mass spectrometry MALDI-TOF, matrix assisted laser desorption ionization/time of flight ESI, electrospray icmization PVP, polypyrolidone TFA, trifluoroacetic acid. 

MALDI-TOF = Matrix-assisted Laser Desorption Ionization Time-of-flight... 

Different mass spectrometric methods are available. For dendrimers, the main ones are those that are able to ionize the samples gently and that can be used with high molecular weight compounds, e.g. MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) and ESI (electrospray ionization). These methods have allowed evaluation of the purity of dendrimers such as PPI and PAMAM. However, precautions must be taken in the interpretation of purity data as the different matrices or ionization techniques can modify the dendrimer structure and lead to misinterpret-... 

Figure 7.3 K Ras4B C-terminal peptide was synthesized employing the trityl linker and after purification was reacted with a K-Ras thioester (K-Ras 1-174 MesNa) via EPL, yielding the semisynthetic K-Ras4B. Purity and identity of the purified protein were characterized by SDS-PAGE and MALDi-TOF. SDS-PAGE sodium dodecyl sulfate/polyacrylamide gel electrophoresis, MALDi-TOF matrix-assisted laser desorption ionization/time of flight.

---