Luna Phenyl-Hexyl

Koal et al. (2004) measured four immunosuppressants (cyclosporine A, tacrolimus, sirolimus, and everolimus) in whole blood samples from transplant recipients. The samples were treated first with a protein precipitation step. The supernatant was extracted with a Poros Rl/20 perfusion column (30 x 2.1 mm, 20 tm, Applied Biosystems, Darmstadt, Germany) online. A Luna phenyl hexyl column (2 x 50 mm, Phenomenex, Schaffenburg, Germany) was used for separation. The total run time was 2.5 min. The lower limit of quantitation was 10 ng/mL for cyclosporine A and 1 ng/mL for the other three analytes. 

Figure 1 Separation of impurities A, B, and C from the peak of interest (P) using seven different HPLC systems. Reprinted from [14], copyright 2004, with permission from Elsevier. (For each system the column temperature is 30°C, the detector is UV 254 nm and the gradient is a 60-min gradient from 5% to 95% organic modifier. Ml column 250 X 4.6 mm i.d. 5 pm Kromasil C4, mobile-phase acetonitrile/0.1% trifluoroacetic acid [pH 1.9] M2 column 100X4.6 mm i.d. 5 pm Luna phenyl-hexyl, mobile-phase acetonitrile/0.1% acetic acid adjusted to pH 3.5 with ammonium hydroxide M3 column 100 X 4.6 mm i.d. 5 pm Luna phenyl-hexyl, mobile-phase acetonitrile/10 mM ammonium acetate [pH 7.0] M4 column 100 X 4.6 mm i.d. 5 pm Luna phenyl-hexyl, mobile phase THF/10 mM ammonium acetate adjusted to pH 5.0 with glacial acetic acid M5 column 150 X 4.6 mm i.d. 3 pm Spherisorb ODSl, mobile-phase methanol/10 mM ammonium acetate [pH 7.0] M6 column 150 X 4.6 mm i.d. 5 pm Monitor Cl8, mobile-phase methanol/0.1% acetic acid adjusted to pH 3.5 with ammonium acetate M8 column 100 X 4.6 mm i.d. 4 pm YMC J Sphere ODS H80, mobile-phase acetonitrile/0.1% formic acid [pH 2.1] [M7 is a variation on the M8 gradient and is not shown].)...

Separation of five compounds (DL, 6-OH-DL, 3-OH-DL, 7V-OH-DL, and 1-pyridine-/V-oxide-DL) was achieved using an Alliance HPLC system (Waters Corp., Milford, CA) equipped with a 2690 model pump, an autoinjector, a Polaris Cl8-A guard column (Varian Inc., Lake Forest, CA), and a Luna Phenyl-Hexyl analytical column (Phenomenex, Inc., Torrance, CA) maintained at 40°C. For robust characterization of each isomeric compound, an online HDX LC-MS method was developed. The composition of regular and deuterated mobile phases is summarized below ... 

Phenomenex Luna phenyl-hexyl Agilent Zorbax Bonus RP and Extend C18 YMC Pro C 18... 

Analytical column SymmetryShield RP8 Luna Phenyl-Hexyl... 

Rooibos tea from Aspalathus linearis 4 flavones, 8 flavonols, 2 dihydrochalcones SLE (boiled, water) Phenomenex Luna Phenyl-Hexyl (250 X 4.6 mm, 5 pm) Graditmt 2 % acetic acid (aq.) and MeCN DAD [123]... 

SynergiMAX RP/SynergiHydro, Jupiter, SynergiFUSION RP, SynergiPOLAR RP, Luna Phenyl Hexyl (LiChrospher, in alkaline pH Gemini)... 

The samples (50 (jul) were analysed on a Hewlett Packard 1050 DAD system upgraded with a 1090 DAD optical bench. The column was a Luna 5 j,m phenyl-hexyl, 250 mm x 4.6 mm maintained at 30 °C. The flow rate was 1 ml min-1 using a gradient elution of acetonitrile and water as follows ... 

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