Luna Phenyl

Koal et al. (2004) measured four immunosuppressants (cyclosporine A, tacrolimus, sirolimus, and everolimus) in whole blood samples from transplant recipients. The samples were treated first with a protein precipitation step. The supernatant was extracted with a Poros Rl/20 perfusion column (30 x 2.1 mm, 20 tm, Applied Biosystems, Darmstadt, Germany) online. A Luna phenyl hexyl column (2 x 50 mm, Phenomenex, Schaffenburg, Germany) was used for separation. The total run time was 2.5 min. The lower limit of quantitation was 10 ng/mL for cyclosporine A and 1 ng/mL for the other three analytes. 

Figure 1 Separation of impurities A, B, and C from the peak of interest (P) using seven different HPLC systems. Reprinted from [14], copyright 2004, with permission from Elsevier. (For each system the column temperature is 30°C, the detector is UV 254 nm and the gradient is a 60-min gradient from 5% to 95% organic modifier. Ml column 250 X 4.6 mm i.d. 5 pm Kromasil C4, mobile-phase acetonitrile/0.1% trifluoroacetic acid [pH 1.9] M2 column 100X4.6 mm i.d. 5 pm Luna phenyl-hexyl, mobile-phase acetonitrile/0.1% acetic acid adjusted to pH 3.5 with ammonium hydroxide M3 column 100 X 4.6 mm i.d. 5 pm Luna phenyl-hexyl, mobile-phase acetonitrile/10 mM ammonium acetate [pH 7.0] M4 column 100 X 4.6 mm i.d. 5 pm Luna phenyl-hexyl, mobile phase THF/10 mM ammonium acetate adjusted to pH 5.0 with glacial acetic acid M5 column 150 X 4.6 mm i.d. 3 pm Spherisorb ODSl, mobile-phase methanol/10 mM ammonium acetate [pH 7.0] M6 column 150 X 4.6 mm i.d. 5 pm Monitor Cl8, mobile-phase methanol/0.1% acetic acid adjusted to pH 3.5 with ammonium acetate M8 column 100 X 4.6 mm i.d. 4 pm YMC J Sphere ODS H80, mobile-phase acetonitrile/0.1% formic acid [pH 2.1] [M7 is a variation on the M8 gradient and is not shown].)...

Resolve C18, Nomura Devosil ODS-MG-5, YMC AQ312, Platinum EPS, Luna Phenyl C6 and Luna phenyl C8) were compared with slight modification in the mobile phase gradient and the Luna Phenyl C8 resulted the most selective and robust [609],... 

Separation of five compounds (DL, 6-OH-DL, 3-OH-DL, 7V-OH-DL, and 1-pyridine-/V-oxide-DL) was achieved using an Alliance HPLC system (Waters Corp., Milford, CA) equipped with a 2690 model pump, an autoinjector, a Polaris Cl8-A guard column (Varian Inc., Lake Forest, CA), and a Luna Phenyl-Hexyl analytical column (Phenomenex, Inc., Torrance, CA) maintained at 40°C. For robust characterization of each isomeric compound, an online HDX LC-MS method was developed. The composition of regular and deuterated mobile phases is summarized below ... 

Phenomenex Luna phenyl-hexyl Agilent Zorbax Bonus RP and Extend C18 YMC Pro C 18... 

Analytical column SymmetryShield RP8 Luna Phenyl-Hexyl... 

Rooibos tea from Aspalathus linearis 4 flavones, 8 flavonols, 2 dihydrochalcones SLE (boiled, water) Phenomenex Luna Phenyl-Hexyl (250 X 4.6 mm, 5 pm) Graditmt 2 % acetic acid (aq.) and MeCN DAD [123]... 

SynergiMAX RP/SynergiHydro, Jupiter, SynergiFUSION RP, SynergiPOLAR RP, Luna Phenyl Hexyl (LiChrospher, in alkaline pH Gemini)... 

The samples (50 (jul) were analysed on a Hewlett Packard 1050 DAD system upgraded with a 1090 DAD optical bench. The column was a Luna 5 j,m phenyl-hexyl, 250 mm x 4.6 mm maintained at 30 °C. The flow rate was 1 ml min-1 using a gradient elution of acetonitrile and water as follows ... 

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