Agilent Zorbax

Salm et al.44 developed a high-throughput analytical method to measure cyclosporine in whole blood. They used a simple SPE procedure, followed by HPLC-MS/MS. An Agilent 1100 liquid chromatograph was coupled with an Agilent Zorbax Bonus C18 reversed-phase column (50 x 2.1 mm, 5 jt/rn particle size). The column temperature was maintained at 70°C in a column oven. The mobile phase consisted of 80% methanol and 20% 40mM ammonium acetate buffer (pH 5.1) delivered isocratically at a flow of 0.4 mL/min. D12 cyclosporine was the IS. 

Figure 2-14. Retention of alkylbenzenes (benzene-octylbenzene) on Agilent Zorbax-Eclipse XDB C18 colnmn from different composition of acetonitrile/water eluent. Left pane Retention volumes right pane Retention factors.

B. G. Beienkii and L. Z. Vilenchik, Modern Liquid Chromatography of Macromolecules, Journal of Chromatography Library, voi. 25, Elsevier Amsterdam, 1983. Agilent Zorbax PrepHT GF-250 datasheet, Agilent Technologies, 2004. 

Phenomenex Luna phenyl-hexyl Agilent Zorbax Bonus RP and Extend C18 YMC Pro C 18... 

LC/MS/MS Analysis The samples (75 pL) are loaded onto an Agilent Zorbax RX-C8 column (4.6 mm x 250 mm, 5 pm, Wilmington, DE). The flow rate is set at 1 mL/min with a 1 5 split to the mass spectrometer ion source and a waste container, respectively. The mobile phase consists of solvent A (5 mM ammonium acetate in water-acetonitrile-acetic acid, 95 5 0.05, v/v/v) and solvent B (5mM ammonium acetate in acetonitrile-water-acetic acid, 95 5 0.05, v/v/v). The HPLC runs are programmed by a linear increase from 0% to 80% of solvent B during a 30 min period. The mass spectra are recorded in full scan and data-dependent scans of MS" (n = 2, 3, or 4). The MS" spectra... 

Rongjie, R, Jianzhong, L., and Wang, Y. 2010. Fat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C18 Bonded Colurtm. Application Note. 

Anthocyanins Agilent Zorbax SB-Cl 8 analytical (5 5 % formic acid in water - methanol/ DAD 520 [11]... 

Black currant juice 3 llavones, 2 flavonols, 3 flavanones None (direct injection) Agilent Zorbax Rapid Resolution Ci8 column (50 X 2.1 mm, 1.8 pm) Gradient 0.1 % formic acid (aq.) and 0.1 % formic acid in MeCN MS (—ve, LTQ-Orbitrap, MS ) [118]... 

Citrus grandis, Citrus paradise (flavedos external layer of peel and juices) 15 flavonols, 13 flavanones LLE (sonication, MeOH) Agilent Zorbax SB Ci8 column (250 X 4.0 mm, 5 pm) Gradient 1 % acetic acid (aq.) and 1 % acetic acid in MeCN DAD-MS (—ve. Ion trap, MS ) [122]... 

Agilent Zorbax Eclipse XDB-C18 (4.6x50 mm, 1.8 pm) (Agilent, Mississauga, ON, Canada) with 0.2 pm stainless steel flit guard assembly (Waters Acquity UPLC part 205000303). 

The separation employed an Agilent Zorbax, RX-C18 column (4.6 mm i.d. x 15 cm, 5 pm particle size) (Wilmington, DE, USA) and the mobile phase consists of acetonitrile and 0.1% TEA in the ratio 38 62 v/v, filtered through a nylon membrane and degassed under vacuum before use. The column compartment was maintained at 25°C. Using a 50 pL injection volume, the analytes were monitored with UV detection at 210 nm for a total runtime of 20 minutes at a flow rate of 1.0 mL/min. 

Agilent Zorbax Eclipse Pins C18 3.5 or 5 p,m Zorbax Eclipse Plus Cl8 RRHD 1.8 p,m... 

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