Protein Lowry assay

Basic Protocol 1 The Lowry Protein Assay for Determination of Total Proteins B1.1.3 Alternate Protocol 1 Modified Lowry Protein Assay for Determination... 

Figure B1.1.1 The reaction schematic for the Lowry Protein Assay.

THE LOWRY PROTEIN ASSAY FOR DETERMINATION OF TOTAL PROTEINS... 

Modified Lowry Protein Assay Kit (Pierce) containing ... 

Table B1.1.1 Troubleshooting Guide for the Modified Lowry Protein Assay... 

Figure B1.1.2 Graph of the color response curves obtained with Pierce s Modified Lowry Protein Assay Reagent using bovine serum albumin (BSA) and bovine gamma globulin (BGG). The standard tube protocol was performed and the color was measured at 750 nm in a Hitachi U-2000 spectrophotometer.

Use the BCA (see Basic Protocol 2) or Lowry protein assay (see Basic Protocol 1)... 

The protein-to-protein variation in the amount of color produced with the BCA Protein Assay Reagent (CV = 15% for the group of 14 proteins at 1000 pg/ml in the standard tube protocol) is similar to that observed for the modified Lowry protein assay reagent. 

Figure B1.1.2 shows the color response curves obtained with the Modified Lowry Protein Assay Reagent using BSA and BGG. The graph shows the net absorbance at 750 nm versus the protein concentration at seven concentrations from 125 to 2000 pg/ml for each protein. Note that the response curve for BGG is higher than the response curve for BSA.

Quantification of erythrocyte membranes and cytoskeletons. The Lowry protein assay... 

Protein Specitic Staining Curves Plots ot silver stain densities versus protein concentrations produce ditterent staining curves tor each proteins studied (16.24.25.32). Protein specitic staining curves have also been observed with the organic stains, including Coomassie Blue (51,72) and with most protein assays such as the commonly used Lowry protein assay (7 1.). These curves are governed by the basic... 

In any case, you should not approach papers as if they hold the holy revelation. Even the Lowry Protein Assay has not been examined and optimized in every direction, and a protocol that is optimal for protein 1 may not be so for protein 2. Treat methods circumspectly. Question them (why phosphate buffers, why incubate before with X-ase ). Upon first application it is actually advisable to follow a protocol exactly, but later a playful interaction with the recipe is more usefiil. Also, you should be wary of all assertions regarding advantages, speed, or sensitivity of the tests or methods. On the other hand, warnings about disadvantages should be taken seriously. Researchers write papers not to assist their colleagues but to receive their recognition and to extend their publication list. The tone of the EXPERIMENTER is pointedly unacademic. 

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