Lodoacetic acid

Hpall is a restriction endonuclease Helix-tum-helix High-throughput screening Haplotype single-nucleotide polymorphism Human genome organization Human umbilical vein endothelial cells lodoacetic acid... 

Carboxymethylation 50 pL of 10 mM iodoacetamide (made up fresh in 100 mM ammonium bicarbonate in 10% acetonitrile) is added and the tube incubated for 30 min at room temperature in the dark. The supernatant is removed and the gels washed as follows. This step is performed to carboxymethylate the cysteine residues to ensure that disulfide bonds cannot re-form. This increases the molecular weight of the residue by approx 57 Da and needs to be taken into account when the database search is performed (4). lodoacetic acid can be employed, rather than iodoacetamide, but this causes an increase of approx 58 Da in the weight of cysteine residues. 

SYNS lODOACETATE SODIUM SALT lODOACETIC ACID SODIUM SALT USAF EK-6279... 

Materials Escherichia co/i-derived rhSCF (SDS-dissociable dimer) was purified according to methods described previously (12,13). The recombinant molecule contains 165 amino acids plus an N-terminal methionine at position -1. lodoacetic acid was purchased from Sigma. HPLC solvents and water were purchased from Burdick and Jackson. Sequencing reagents and solvents were supplied by Applied Biosystems (Foster City, CA) and Hewlett Packard (Mountain View, CA). All other reagents were of the highest quality available. 

Ethyl iodoacetate lodoacetic acid, ethyl ester KSK Reagent Experimental... 

---