Lobster Spiny, Panulirus

The deoxy forms of hemocyanins are colorless, as a result of their 3d ° dicopper(I) centers. Although chemical and x-ray absorption spectroscopic studies had shed considerable light on the nature of the deoxy-He dicopper binding site, there now exist two x-ray crystal structures, the first on the the spiny lobster He, Panulirus interruptus [23], and a recent one of the horseshoe crab Limulus II protein [24], The structures exhibit rather different active-site characteristics, and since the former was crystallized at low pH and possesses rather odd copper coordination, the latter Limulus II structure is probably representative. It indicates that the two Cu(I) ions are 4.6 A apart, each found in a trigonal-planar coordination environment with Cu-NMs bond distances of about 2.0 A (Figure 1). Intersubunit 02 binding cooperative effects are probably initiated and trans-... 

American lobster, Homarus americanus serum Marine crustaceans Muscle, 10 species Whole, various species Aesop shrimp, Pandalus montagu r, soft parts Scotland reference site vs. waste dump site Caribbean spiny lobster, Panulirus argus soft parts Puerto Rico Anasco Bay West coast... 

Reeder, P. B. and B. W. Ache. Chemotaxis in the Florida spiny lobster, Panulirus argus. Anim. Behav. 28, 831-839 (1980). 

Zimmer-Faust, R.K. and Case, J.F., A proposed dual role of odor in foraging by the California spiny lobster, Panulirus inlerruptus (Randall), Biol. Bull., 164, 341, 1983. 

Crustaceans are known to use pheromones in behavioral contexts other than avoidance, including reproduction and social interactions. For example, at least one spiny lobster, the California spiny lobster Panulirus interrupts, has been shown to be attracted to the odor of conspecifics.131 This pheromonal phenomenon is taken advantage of by workers in the lobster fishery, who use live lobsters as bait in their pots.132 However, relatively little research has been performed on the topic of aggregation pheromones, including the nature of the signal and its sensory reception. Pheromonal chemical signals are also involved in the establishment and maintenance of social hierarchies in crustaceans.133 134... 

Johnson, B. R. and Ache, B. W., Antennular chemosensitivity in the spiny lobster, Panulirus argus amino acids as feeding stimuli, Mar. Behav. Physiol., 5, 145, 1978. 

Olson, K. S. and Derby, C. D., Inhibition of taurine and 5 AMP olfactory receptor sites of the spiny lobster Panulirus argus by odorant compounds and mixtures, J. Comp. Physiol, A, 176, 527, 1995. 

Burgess, M. F. and Derby, C. D., Two novel types of L-glutamate receptors with affinities for NMDA and L-cysteine in the olfactory organ of the Caribbean spiny lobster Panulirus argus, Brain Res., 111, 292, 1997. 

Zimmer-Faust, R. F., Tyre, J. E., and Case, J. F., Chemical attraction causing aggregation in the spiny lobster, Panulirus interruptus (Randall), and its probable ecological significance, Biol. Bull., 169, 106, 1985. 

Hunt, J. H., Lyons, W. G., and Kennedy, F. S., Effects of exposure and confinement on spiny lobsters, Panulirus argus, used as attractants in the Florida trap fishery, Fish. Bull., 84, 69, 1985. 

Gleeson R. A., Trapido-Rosenthal H. G., McDowell L. M., Aldrich H. C. and Carr W. E. (1992) Ecto-ATPase/phosphatase activity in the olfactory sensilla of the spiny lobster, Panulirus argus localization and characterization. Cell Tissue Res. 269, 439-445. 

Chinese spiny lobster (Panulirus stimpsoni) Pan s 1 Crustaceans Tropomyosin 34 cDNA Yes Leung et al. (1998b)... 

Figure 2 Active sites of hemocyanins from horseshoe crab L. polyphemus (a oxy b deoxy), spiny lobster Panulirus interruptus (c), sweet potato Ipomoea batatas (d), Octopus dofleini (e), Rapana thomasiana (f). Cleary the four alpha-helix bundle motif with the metal center can be seen. The three histidines coordinating Cu-A are colored red, those coordinating Cu-B green. The two copper atoms are colored blue and oxygen red. The cysteines binding covalently a histidine at the Cu-A site are colored yellow. In the case of Ipomoea, a water molecule connects the two copper atoms...

There are two species of spiny lobsters in North America. The West Coast spiny lobster Panulirus inter-ruptus) occurs on the Pacific coast, and the Caribbean spiny lobster (P. argus) occurs in the Caribbean Sea, off the Horida Coast, and in the Gulf of Mexico. These warm-water species do not have the huge, crushing, and tearing claws of the Homarus typical lobsters, but they have a needle-sharp, spiny carapace, and very long antennae. 

D.R. Cummins, D.M. Chen, T.H. Goldsmith (1984). Spectral sensitivity of the spiny lobster Panulirus argus. Biol. Bull, 166, 269-276. 

Crustacea Decapoda Panulirus interruptus (subunit type a) (spiny lobster) 29.6 11.3 0.8... 

The fates of the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) 2, 4, 5-trichlorophenoxyacetic acid (2,4,5-T) and the DDT metabolite, bis-(4-chlorophenyl) acetic acid (DDA) were studied in the spiny lobster, Panulirus argus 24). All acids were injected intrapericardially at 10 mg/kg. Both 2,4-D or 2,4,5-T were extensively excreted, unchanged, in urine in the first 24 hours after the dose. About 10% of the dose of 2,4,D and 2,4,5T was taken up by hepatopancreas where the taurine conjugate was formed and subsequently excreted either in urine or feces. Part of the DDA dose was excreted in urine, as unchanged DDA and as DDA-taurine, but DDA was more extensively taken up by shell and hepatopancreas and more slowly excreted than 2,4D or 2,4,5-T. For all three carboxylic acids, muscle concentrations were lower than hemolymph concentrations at 24 hours after the dose, and were less than 5p.g/g. 

Fig. 28.8 The structure of deoxyhaemocyanin from the spiny lobster Panulirus interruptus) (a) the backbone of the protein chain and the positions of the two Cu(I) centres, and (b) the active site in which the two Cu(I) centres are bound by histidine residues, (c) The 02-binding site in oxyhaemocyanin from the Atlantic horseshoe crab (Limulus polyphemus). Hydrogen atoms are omitted colour code Cu, brown C, grey O, red N, blue.

Kanazawa, A. and Teshima, S., 1971. In vivo conversion of cholesterol to steroid hormones in the spiny lobster, Panulirus japonica. Bull. Jap. Soc. Sci. Fish., 27 207—212. 

Hemocyanins (Hcl are oxygen-transport nonheme proteins I MW 10 lO ) which are found in the blood of some insects, crustaceans, and other invertebrates. One of the smallest He (MW 450,000) e.xiracted from spiny lobster Panulirus inierrupius consists of six subunits each containing two Cu atoms. Upon oxygenation, the deoxy form (Cu(I), colorless) turns to blue (Cu(ll), blue blood ) by binding one O2 molecule per two Cu atoms. 

Homer AJ, Weissburg MJ, Derby CD (2008) The olfactory pathway mediates sheltering behavior of Caribbean spiny lobsters, Panulirus argus, to conspecific urine signals. J Comp Physiol A 194 243-253... 

Ziegler TA, Forward RB (2007) Larval release behaviors in the Caribbean spiny lobster, Panulirus argus role of peptide pheromones. J Chem Ecol 33 1795-1805... 

Fig. 5.2 Diagram of the spiny lobster, Panulirus argus, flicking the aesthetasc-bearing lateral filaments of its olfactory antennules. Drawing by Jorge A. Varela Ramos...

Reidenbach MA, George NT, Koehl MAR (2008) Antennule morphology and flicking kinematics facilitate odor sampling by the spiny lobster, Panulirus argus. J Exp Biol 211 2849-2858 Sainte-Marie B (1986) Feeding and swimming of lysianassid amphipods in a shallow cold-water... 

Cate HS, Derby CD (2001) Morphology and distrbution of setae on the antennules of the Caribbean spiny lobster Panulirus argus reveal new types of bimodal chemo-mechanosensilla. Cell Tissue Res 304 439-454... 

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