Liquid chromatography pantothenic acid

More recently [635], a unique extraction step in supplemented foods, by using hot water and a precipitation solution, following by HPLC-ELD/UV analysis has been performed for the simultaneous determination of pyridoxine, thiamine, riboflavin, niacin, pantothenic acid, folic acid, cyanoco-balamin, and ascorbic acid. The mobile phase consisting of phosphate buffer and methanol has been modified in order to perform ion-liquid chromatography by adding l-octanesulfonic acid sodium salt. Furthermore, triethylamine has been also added to improve peak symmetry. 

JM Romera, M Ramirez, A Gil. Determination of pantothenic acid in infant milk formulas by high performance liquid chromatography. J Dairy Sci 79 523 -526, 1996. 

Rychlik, M., 2003. Pantothenic acid quantification by a stable isotope dilution assay based on liquid chromatography-tandem mass spectrometry. Analyst. 128 832-837. 

Takahashi, K., Fukuwatari, T., and Shibata, K., 2009. Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column deri-vatization. Journal of Chromatography B. 877 2168-2172. 

High-performance Liquid Chromatography Mass Spectrometry Analysis of Pantothenic Acid (Vitamin Bg) in Multivitamin Dietary Supplements... 

Review of Analysis of Pantothenic Acid Using High-performance Liquid Chromatography... 

In the last two decades high-performance liquid chromatography (HPLC) has become a valuable method for the separation and quantitation of pantothenic acid and some of its derivatives. Several methods for the determination of pantothenic acid and its salts (mainly calcium pantothenate), or panthenol, in pharmaceutical preparations have been developed and are applied on a routine basis in a few research and control laboratories (50-52). 

Pantothenic acid, its salts, and panthenol as such are not volatile enough for direct gas-liquid chromatography (GLC). However, it is possible to use this chromatographic technique after derivatization of the polar hydroxyl and carboxyl groups of the vitamin (23,38,72,73,75-79). The majority of the developed methods are, however, applicable only to relatively pure and simple samples such as multivitamin preparations, and certain biological samples, such as urine (75-77). Only a few methods are suitable for the determination of the vitamin in complex matrices such as foods. An overview of methods was given by Velisek et al. (5). 

T Aral, H Matsuda, H Oizumi. Determination of optical purity by high-performance liquid chromatography on chiral stationary phases pantothenic acid and related compounds. J Chromatogr 474 405-410, 1989. 

Pakin C, Bergaentzle M, Hubscher V, Aoude-Werner D, Hasselmann C. Fluorimetric determination of pantothenic acid in foods by liquid chromatography with postcolumn derivatization. J Chromatogr A 2004 1035(1) 87—95. 

---