Liquid chromatography/mass spectrometry tubing

Queiroz ME, Oliveira EB, Breton F et al (2007) Immunoaffinity in-tube solid phase microextraction coupled with liquid chromatography-mass spectrometry for analysis of fluoxetine in serum samples. J Chromatogr A 1174 72-77... 

Wu, J., Tragas, C., Lord, H., and Pawliszyn, J. (2002). Analysis of polar pesticides in water and wine samples by automated in-tube solid-phase microextraction coupled with high-performance liquid chromatography-mass spectrometry, J. Chromatogr. A, 976,357-367. 

In most cases, the thermal decompositions lead to a coevolution of several materials, particularly when the backbone of the polymer is broken apart. Because of the mbcture of products generated, identification of the constituents could only be made through the use of spectral subtraction or when small molecules with simple infrared spectra, such as carbon monoxide, carbon dioxide, and hydrogen chloride were generated. In the study on polyacrylamide (2), the effluent was trapped in a gas collection tube immersed in liquid nitrogen and then separated and analyzed by GC/FT-IR and gas chromatography/mass spectrometry. 

J. C. Wu, Z. Mester, J. Pawliszyn, Speciation of organoarsenic compounds by polypyrrole-coated capillary in tube solid phase microextraction coupled with liquid chromatography/electrospray ionisation mass spectrometry, Anal. Chim. Acta, 424 (2000), 211-222. 

Imoto et al. by high performance liquid chromatography combined with atmospheric pressure ionisation mass spectrometry (API-MS). The crude saponin isolated from the leaves was chromatographed on octadecyl silica column and eluted with an aqueous methanol solution containing ammonium acetate. The fractions thus separated were directly introduced into an atmospheric pressure ionisation mass spectrometer connected with the liquid chromatograph by an interface consisting of a nebulizer and a vaporizer through a PTFE tube (Hitachi, Japan). The vaporized sample and solvent molecules at 300°C were introduced into the ion source of the atmospheric pressure ionisation system. 

Kataoka, H. and Pawliszyn, J., Development of in-tube solid-phase microextraction/liquid chromatography/electrospray ionization mass spectrometry for the analysis of mutagenic heterocyclic amines, Chromatographia, 50(9/10), 532-538, 1999. 

Takino, M., Daishima, S., and Nakahara, T., Automated online in-tube solid-phase microextraction followed hy liquid chromatography/electrospray ionization-mass spectrometry for the determination of chlorinated phenoxy acid herbicides in environmental water. Analyst, 126, 602-608,... 

Automated in-tube solid-phase microextraction (SPME) has recently been coupled with liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS), e. g. for the determination of drugs in urine [60, 62]. In-tube SPME is an extraction technique in which analytes are extracted from the sample directly into an open tubular capillary by repeated draw/eject cycles of sample solution. The analyte is then desorbed with methanol and transferred to an analytical HPLC-column. 

DCIM-MS has been coupled to liquid chromatography (LC) for analyzing complex peptide samples [36], Peptides eluting from the LC column were analyzed on an IMS-Q-TOF mass spectrometer. The ions generated from the source were accumulated in an ion trap and injected periodically into the drift tube. After mass analysis by the quadrupole, ions were subjected to collision-induced dissociation (CID) within an octopole collision cell and the product ions were analyzed by a TOF analyzer. Using this instrumental configuration, the urinary proteome [37], the Drosophila melano-gaster head proteome [38], and the human plasma proteome [39] have been analyzed. While many additional measurements, compared to standard mass spectrometry-based proteomics experiments, were obtained (for example, collision cross-sections), these were not used to improve upon protein identification results. 

Samples analyzed by El mass spectrometry must be converted to gas phase. For pure gases or volatile liquids the samples may be introduced directly through a small orifice that allows an appropriate amount of material into the vacuum chamber. A small amount of a solid sample can be placed in a melting point capillary tube and inserted into the mass spectrometer at the end of a metal rod, called a direct insertion probe (DIP).The temperature at the tip of the probe can be varied to promote sublimation of the sample. Another common method of sample introduction is gas chromatography, which is the ideal choice for samples that are impure. 

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