Liquid chromatography future trends

This chapter presents an overview of current trends in high-pressure liquid chromatography (HPLC) instrumentation focusing on recent advances and features relevant to pharmaceutical analysis. Operating principles of HPLC modules (pump, detectors, autosampler) are discussed with future trends. 

Some idea of the future trend of methylation analysis can, perhaps, be gained from the work of Perila and Bishop, who have used the method to study oligosaccharides obtained by the enzymic hydrolysis of jack-pine mannan. The oligosaccharides (0.5-2.0 mg.) were methylated by the Kuhn procedure, refluxed with methanolic hydrogen chloride, and the resulting methyl 0-methylglycosides analyzed qualitatively and quantitatively by gas-liquid chromatography. 

For both. A- and B- trichothecenes there is still a lack of simple and reliable screening methods enabling the rapid detection of these mycotoxins at low cost. Besides the increasing demand for rapid screening methods for both A- and B-trichothecens, the use of liquid chromatography with tandem mass spectrometry (LC-MS/MS) enabling both quantification and identification of several trichothecenes simultaneously can be considered a major future trend in the analysis of these Fusarium mycotoxins in cereals (Krska et al., 2001). 

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