Lipopolysaccharides Klebsiella

Structural studies on the oligosaccharide derivatives obtained by partial, acid hydrolysis of fully methylated polysaccharides often furnish valuable information on the positions at which the oligosaccharides were linked in the original polysaccharide. When the folly methylated Klebsiella O group 9 lipopolysaccharide,27 which contains D-galactopyranose and D-galactoforanose residues, was subjected to mild, acid hydrolysis, and the product reduced with lithium aluminum deuteride and remethylated with trideuteriomethyl iodide, a good yield of the disaccharide derivative 10 was obtained. 

The method has also been applied to acetylated polysaccharides. Thus, whereas the D-mannopyranose residue in the acetylated lipo-polysaccharide from Salmonella typhi was found to be inert, that in Salmonella Strasbourg was oxidized, demonstrating that the former is a-D-linked and the latter /3-D-linked.154 The D-mannose residues in the acetylated lipopolysaccharide from Klebsiella O-group 3 were found resistant to oxidation, and are consequently a-D-linked."... 

The O-antigen polysaccharides of Klebsiella serotype 05 and Escherichia coli 08 were prepared by mild hydrolysis of the lipopolysaccharides. A bacteriophage enzyme hydrolyzed both polymers,3150 giving the trisaccharide /3-D-Man-(l - 2)-a-D-Man-(l -> 2)-D-Man. 

It is necessary to postulate the existence of a previously unknown, modification reaction of glycosyl nucleotides, that is, deoxygenation at C-4, in order to explain the formation of 4-deoxy-D-arafc//io-hexose, a monosaccharide constituent of Citrobacter lipopolysaccharide.202 4-Deoxy-L-threo-hex-4-enuronic acid, found in Klebsiella K22 polysaccharide,203 may be formed through dehydration of the D-galacturonic acid group or residue, either in the glycosyl nucleotide, or in the polysaccharide. 

Whitfield, UDP-galactofuranose precursor required for formation of the lipopolysaccharide O antigen of Klebsiella pneumoniae serotype 01 is synthesized by the product of the rfbD(KPOl) genej. Biol. Chem. 1997, 272, 4121-4128. 

Mana 1 -3 Mana 1 -2Mana 1 -2Mana 1 -2aMana 1 -Klebsiella 03 lipopolysaccharide... 

Methyl Ethers" from the Hydrolyzates of Methylated Lipopolysaccharides from Klebsiella 0-group 9... 

In structural studies on Klebsiella O-group 5 lipopolysaccharides by the Smith-degradation technique, 2-deoxy-glycerol-2-yl a-n-mannopyrano-side was obtained. This disaccharide was characterized, as its trimethyl-silyl derivative, by g.l.c.-m.s. 

B. Lindberg, J. Lonngren, W. Nimmich, and U. Ruden, Structural studies on the Klebsiella O group 7 lipopolysaccharide, Acta Chem. Scand., 27 (1973) 3787-3790 B. Erbing, B. Lindberg, and S. Svensson, Smith degradation, Acta Chem. Scand., 28 (1974) 1180-1184. 

Tomas JM, Camprubi S, Merino S ct al. Surface exposure of 01 serotype lipopolysaccharide in Klebsiella pneumoniae strains expressing difierent K antigens. Infect Immun 1991 59 2006-2011. 

Constit. of lipopolysaccharide in Klebsiella coli. Also in mannan of Saccharomyces rouxii and prod, by Streptomyces griseus. Cryst. (EtOH/EtjO). 

The O-specific side-chains in Klebsiella O group 12 lipopolysaccharide are composed of the disaccharide repeating-unit (9) joined by (1 3)-linkages. Two mutants that lack both the capsular and lipopolysaccharide O antigens... 

Wilkinson JF, Stark GH (1956) The synthesis of polysaccharide by washed suspensions of Klebsiella aerogenes. Proc Roy Phys Soc Edinburgh 25 35-39 Williams ST, Sharpe ME, Holt JG (1989) Bergey s manual of systematic bacteriology. Williams and Wilkins, Baltimore, http //www.cme.msu.edu/bergeys Williams V, Fletcher M (1996) Pseudomonas fluorescens adhesion and transport through porous media are affected by lipopolysaccharide composition. Appl Environ Microbiol 62 100-104... 

Olsthoorn, M. M. A. Haverkamp, L TIiomas-Oaies, 1. E. Mass spectrometric analysis of Klebsiella pneumoniae ssp. pneumoniae rough strain R20 (01 (-) K20(-)) lipopolysaccharide preparations Identification of novel core oUgosaccharide components and three 3-deoxy-D-monno-oct-2-ulopyranosonic artefacts. J. Mass Spectrom. 1999,34, 622-636. 

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