Lipopolysaccharide structure Klebsiella

Structural studies on the oligosaccharide derivatives obtained by partial, acid hydrolysis of fully methylated polysaccharides often furnish valuable information on the positions at which the oligosaccharides were linked in the original polysaccharide. When the folly methylated Klebsiella O group 9 lipopolysaccharide,27 which contains D-galactopyranose and D-galactoforanose residues, was subjected to mild, acid hydrolysis, and the product reduced with lithium aluminum deuteride and remethylated with trideuteriomethyl iodide, a good yield of the disaccharide derivative 10 was obtained. 

In structural studies on Klebsiella O-group 5 lipopolysaccharides by the Smith-degradation technique, 2-deoxy-glycerol-2-yl a-n-mannopyrano-side was obtained. This disaccharide was characterized, as its trimethyl-silyl derivative, by g.l.c.-m.s. 

B. Lindberg, J. Lonngren, W. Nimmich, and U. Ruden, Structural studies on the Klebsiella O group 7 lipopolysaccharide, Acta Chem. Scand., 27 (1973) 3787-3790 B. Erbing, B. Lindberg, and S. Svensson, Smith degradation, Acta Chem. Scand., 28 (1974) 1180-1184. 

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