Klebsiella serotypes

A signal at 8C 109.0, displayed17 by a complex heteropolymer obtained from Klebsiella serotype 40, arose from 2,3-di-O-substituted /3-D-galactofuranosyl residues (see Section VI,14). 

The O-antigen polysaccharides of Klebsiella serotype 05 and Escherichia coli 08 were prepared by mild hydrolysis of the lipopolysaccharides. A bacteriophage enzyme hydrolyzed both polymers,3150 giving the trisaccharide /3-D-Man-(l - 2)-a-D-Man-(l -> 2)-D-Man. 

The X-ray diffraction pattern from the sodium salt of the Klebsiella serotype K8 capsule is shown in Figure 5. The... 

Figure 5. Diffraction pattern from the capsular polysaccharide of Klebsiella serotype K8 at 90% relative humidity (provided by Dr. E. D. T. Atkins, University...

The molecular structure of polysaccharides from Klebsiella serotype K8 has been examined by X-ray diffraction and computer-aided molecular modeling. The most favorable molecular model was not the one which had the maximum number of intra-molecular hydrogen bonds. This result suggests that in the analysis one should not a priori choose the molecular model which has the largest number of intra-molecular hydrogen bonds. 

We wish to thank the following people who have most kindly supplied us with various Klebsiella serotypes Professor G.G.S. Dutton, Professor S. Stirm, Dr. J.P. Joseleau,... 

Selective benzoylation (and allylation) of methyl (and benzyl 4-O-benzyl-a-L-rhamnopyranoside (4) has been studied by phase-transfer catalysis and by activation via the tributyl stannate. The former reaction gave the 2,4-dibenzyl ether (5) in high yield, whereas the latter process gave mainly the 3,4-diether (6). An alternative means of obtaining the 2,4-diether (5) involved selective tritylation to give (7) followed by benzylation and removal of the trityl group. These dibenzyl ethers have been employed in the synthesis of the tetrasaccharide repeating unit of the immunodeterminant of Klebsiella serotype K36 polysaccharide. Similar results have been recorded for the selective benzylation of other 4,6-blocked a-mannosides. The syntheses of 3-O-benzyl-D-ribose, 2-0-benzyl-D-ribose, and l,6-anhydro-2,3,2, 4-tetra-0-benzyl-jS-lactose have been recorded. The latter was employed in the synthesis of lacto-A -tetraose. ... 

Constit. of the repeating unit of the capsular antigen of Klebsiella serotype K70. [a]D +10 (H2O). 

Component of bacterial cell walls. Constit. of the repeating unit of the type specific antigen of Streptococcus pneumoniae type 2 and the repeating unit of the capsular antigen of Klebsiella serotype K36 and other bacteria. Amorph. [a]o -48 (c, 0.4 in H2O). 

The use of and natural-abundance n.m.r. spectroscopy in structural investigations of oligo- and poly-saccharides is now commonplace, and these techniques are particularly helpful in assigning the configurations of inter-sugar linkages. They were readily applied to gentiodextrins and their derivatives and Klebsiella serotype and K62 polysaccharides, all of which are com-... 

K62 (23), have been elucidated, mostly using a combination of methylation analysis. Smith degradation, partial hydrolysis with acid, and H n.m.r. spectroscopy. In some cases e.g. Klebsiella serotypes K13, K25, and K37), depolymerization of the polysaccharides was accomplished by glycanase activities associated with particles of Klebsiella bacteriophages. Serological cross-reactions also revealed similarities in the structures of certain capsular polysaccharides for example, the polysaccharide from Klebsiella serotype K13 (18) cross-reacted with that from Klebsiella serotype K2, which has an identical structure except for the absence of the terminal 3,4-0-(l-carboxyethylidene)-P-D-galactopyranosyl residue. 

Sutherland, I. W. (1972). The exopolysaccharides of Klebsiella serotype 2 strains as substrates for phage-induced polysaccharide depolymerases. J Gen Microbiol, 70(2), 331-8. 

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