Linkers oligonucleotides

Fig. 10 Above chemical structure of the PEO-PCl-ssDNA conjugate (with the 22-mer oligonucleotide sequence arm ) and representation of a DNA micelle. Below DNA-controlled attachment of block copolymer micelles on surfaces monitored by measuring the time-dependent frequency and dissipation changes induced by mass adsorption on the QCM sensor in the flow mode. Step 1 addition of the anchor -thiolated oligonucleotide. Step 2 addition of the micelles bearing the linker oligonucleotide, followed by rinsing. Step 3 treatment with the as-arm oligonucleotide (competitive displacement to detach the micelles) [84] (figure adapted with permission of Wiley-VCH)...

Both phosphoramidate and phosphate triester derivatives have been used as linkers to attach reporter groups to oligonucleotides. These derivatives are not entirely resistant to nucleases and they possess a chiral center. They have not been widely iavestigated as antisense dmgs. 

Recent developments in DNA/RNA chemical synthesis have allowed us to attach some functional groups covalently to nucleic acids, thus permitting the introduction of a functionality or properties not normally present in the native biomolecule The use of non-nucleosidic linkers is probably the most popular approach for the 5 -terminal modification of chemically synthesized nucleic acid oligonucleotides and a number of such linkers are commercially available. The linker shown in Fig. 2 is designed as a phosphoramidate derivative so that it can be incorporated into the 5 -terminus of the sequence as the last... 

Preliminary results of DNA sequence detection using the OFRR are thoroughly described by Suter, et al.32 The experimental setup described above for tracking the resonant mode shift is used. An OFRR with RI sensitivity of about 7 nm/RIU was produced and used for these experiments. The OFRR surface was functionalized with 3APS and then DMA was used as an amine-amine crosslinker. The single-stranded oligonucleotide capture probe was synthesized with an amine functional group connected to the 5 end by a 6-carbon linker, and has a 25 base-pair sequence. ... 

Silyl linkers (Tab. 3.5) have successfully been used for the synthesis of natural products [96, 97], oligosaccharides [98], oligonucleotides [99] and in peptide synthesis [100]. 

Fig. 8 Covalent attachment of amine-terminated oligonucleotides to amino-modified (APS) supports via a PDITC linker...

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