Linear polyethyleneimine

Linear polyethyleneimine results only in low yields from low temperature polymerization of ethyleneimine for very long reaction times. It can, however, be synthesized in a targeted manner by polymerization of 2-oxazolines with subsequent hydrolytic cleavage of the resulting polyamides (355,358). 

The polymerization of A/-(2-tetrahydropyranyl)aziridine with subsequent hydrolysis of the resulting polymers has been described as an alternative route for the synthesis of linear polyethyleneimine (359). Linear polyethyleneimine, in contrast to branched polyethyleneimines, is only sparingly soluble in water at room temperature. 

Linear polyethyleneimine is insoluble in benzene, diethyl ether, acetone, and water at room temperature and is soluble in hot water. 

LI can be hydrolyzed to the corresponding polyamine LII. This is the only route to linear polyethyleneimine (m — 2) [Tanaka et al., 1983]. The polymerization of ethyleneimine yields a highly branched and cyclized product. 

In principle, one can obtain an unbranched polyethyleneimine by saponifying the amide groups that are located on the polymer (Example 5-4). It is important to note that the ring-opening polymerization of aziridine does not yield linear polyethyleneimines but rather highly branched polymer structmes. 

The polymer can readily be hydrolized yielding a linear polyethyleneimine (see Example 5-4). 

Poly(styrene-g-ethyleneimine) in the form of macroporous 3% CL beads with linear polyethyleneimine blocks was 42 % quaternized with 1-bromobutane (20)94). 

A major interest, early foreseen [178] of poly(N-acylethyleneimine)s obtained by the cationic polymerization of 2-substituted oxazolines, was their ability to lead to linear polyethyleneimines by hydrolysis reaction. An almost complete basic hydrolysis was performed on polymers prepared using BF3-Et20 catalyst [179]. 

Recently, with perfluoroalkyl derivatives [CF3(CF2)nCO—, n = 2 and 6] a linear polymer with Mn = 3650 was synthesized using 1 % of a protonic acid 65). These substitutents can later be removed by hydrolysis, leaving the linear polyethyleneimine. 

In Vol. I (Adv. Polymer Sci. 37) we have discussed the mechanism of polymerization of thiiranes relative to polymer microstructure. The microstructure influences the properties of the polymers e.g. linear polyethyleneimine is crystalline (mp = 59 °C), whereas the branched one is amorphous. 

Coll JL, Chollet P, Brambilla E, Desplanques D, Behr JP, Favort M. In vivo delivery to tumors of DNA complexes with linear polyethyleneimine. Hum Gene Ther 10 1659-1666. 

FIGURE 12.8 SEM of electrospun scaffold using LTU polymer loaded with a plasmid DNA and linear polyethyleneimine complex. 

Synonyms Linear polyethyleneimine Polyethylenimin Poly (imino (1,2-ethanediyl))... 

Linear 2. See Sodium metaphosphate Linear alcohol C13-15. See C13-15 alcohols Linear CIO alpha olefin. See Decene-1 Linear Cl6 alpha olefin. See Hexadecene-1 Linear CIS alpha olefin. See Octadecene-1 Linear C20 alpha olefin. See Eicosene-1 Linear (C12 and C14) alkyl alcohols, ethoxylated. SeeC12-14 pareth Linear polyethyleneimine. See Polyethylene imine... 

In addition to covalently modified ROMP polymers, noncovalent poly-plexes of cationic ROMP polymers with DNA have been used as transfection agents [218, 219]. Breitenkamp and Emrick showed that PCOE functionalized with cationic polylysine grafts complexed with a plasmid that expresses GFP. This polyplex transfected COS-1 and HeLa cells at comparable or superior levels to commercially available jetPEI (linear polyethyleneimine), SuperFect (dendrimer), and Lipofectamine 2000 (liposomal carrier) transfection vehicles [220]. The ROMP-derived carrier was nontoxic to cells, while all three commercially available reagents led to significant levels of cell death. 

Lower critical solution temperature Linear polyethyleneimine i T-carboxyanhydride Oligonucleotides Poly(e-caprolactone)... 

Saito,Y Higuchi,Y awakami,S Yamashita,F Hashida,M. (2009).Immunostimulatory Characteristics Induced by Linear Polyethyleneimine Plasmid DNA Complexes in Cultured Macrophages, Human Gene Therapy, 20, 137-145. 

Our efforts in this area are concentrated currently on the selective grafting of purine bases and amino acids onto linear polyethyleneimine and related polymeric backbones. We are particularly interested in electron transfer through the side chains. 

B. Brissault, A. Kichler, C. Guis, C. Leborgue, D. Danos, H. Charadame, synthesis of linear polyethyleneimine derivatives for DNA transfection. Bioconjugate Chem. 14 (2003) 581-587. 

Khanam N, Mikoryak C, Draper RK, Balkus KJ (2007) Electrospun linear polyethyleneimine scaffolds for cell growth. Acta Biomater 3(6) 1050-1059. doi 10.1016/j.actbio.2007.06.005... 

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