LightCycler

D.A. Kulesh, R.O. Baker, B.M. Loveless, D. Norwood, S.H. Zwiers, E. Mucker, C. Hartmann, R. Herrera, D. Miller, D. Christensen, L.P. Wasieloski Jr., J. Huggins and P.B. Jahrling, Smallpox and pan-orthopox virus detection by real-time 3 -minor groove binder TaqMan assays on the Roche lightcycler and the Cepheid smart cycler platforms, J. Clin. Microbiol., 42 (2004) 601-609. 

Braham, S., J. Gandhi, S. Beard, and B. Cohen. Evaluation of the Roche LightCycler Parvovirus B19 Quantification Kit for the Diagnosis of Parvovirus B19 Infections. Journal of Clinical Virology 31 no. 1 (2004) 5-10. 

W5. Wittwer, C. T, Ririe, K. M., et al, The LightCycler A microvolume multisample fluorimeter with rapid temperature control. Biotechniques 22(1), 176-181 (1997). 

Hall AG, Hamilton P, Minto L etal. The use of denaturing high-pressure liquid chromatography for the detection of mutations in thiopurine methyltransferase. J Biochem Biophys Methods 2001 47 65-71. Davison JE, McMullin MF, Catherwood MA. Genotyping of thiopurine methyltransferase in patients with acute leukemia using LightCycler PCR. Leuk Lymphoma 2006 47 1624-1628. 

Gutzmer, R. Mommert, S. Kiehl, P. Wittmann, M. Kapp, A. Werfel, T. Detection of clonal T cell receptor gamma gene rearrangements in cutaneous T cell lymphoma by LightCycler-polymerase chain reaction. J. Invest. Dermatol. 2001,116(6), 926-932. 

Nakao, M. Janssen, J. W. Flohr, T. Bartram, C. R. Rapid and reliable quantification of minimal residual disease in acute lymphoblastic leukemia using rearranged immunoglobulin and T-cell receptor loci by LightCycler technology. Cancer Res. 2000, 60(12), 3281-3289. 

Nozaki, A. Kato, N. Quantitative method of intracellular hepatitis C virus RNA using LightCycler PCR. Acta Med. Okayama 2002, 56(2), 107-110. 

Commercial LightCycler-apoB mutation detection kit (Roche Molecular Biochemicals). 

Nauck M, Wieland H, Marz W (2000) Evaluation of the Roche Diagnostics LightCycler-Apo 3500 Mutation Detection Kit. Clin Chem Lab Med 38 667-671... 

Leb V, Stocher M, Valentine-Thon E, Holzl G, Kessler H, Stekel H, et al. Fully automated, internally controlled quantification of hepatitis B Virus DNA by real-time PCR by use of the MagNA Pure LC and LightCycler instruments. J Clin Microbiol 2004 42 585-90. 

Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA> Balis UJ. The Lightcycler a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 1997 22 176-81. 

Kreuzer KA, Lass U, Bohn A, Landt O, Schmidt CA. LightCycler technology for the quantitation of bcr/abl fusion transcripts. Cancer Res 1999 59 3171-4. 

Nakao, M., Janssen, J.W., Seriu, T., and Bartram, C.R. (2000) Rapid and reliable detection of N-ras mutations in acute lymphoblastic leukemia by melting curve analysis using LightCycler technology. Leukemia 14, 312-5. 

For detection of especially dangerous bacterial and viral agents such as Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella species and influenza A/H5, real-time PCR using a LightCycler 2.0 (Roche) and reagents (Idaho Technologies) has been applied at NCDC. 

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