Light spectrometer

A Spectronic 20 (Theromo Scientific, Waltham, MA) with 1-cm diameter sterile glass test tubes works well for this, allowing the %T for the whole suspension to be determined. Alternatively, the %T of an aliquot of suspended bacteria can be determined using a cuvette and a conventional visible light spectrometer set to 650 nm. 

The optofluidic devices can be very effective in microscopic analysis just as typical optical equipment such as UV, IR, Raman, visible light spectrometers, or fluorescence spectrometers are used to analyze molecular information. 

To answer the following questions assume you have access to hydrogen, iodine, hydrogen iodide, a transparent reaction vessel, a visible-light spectrometer, and a means for changing the temperature, (a) Which gas or gases concentration could you readily monitor with the spectrometer (b) To use Beer s law (Equation 14.5) you need to determine the extinction coefficient, e, for the substance in question. How would you determine e ... 

The design and use of spectrographs or spectrometers involves a compromise between resolution—how close in wavelength two lines can be and still be seen as separate—and sensitivity—how weak a light can be observed or how... 

While a laser beam can be used for traditional absorption spectroscopy by measuring / and 7q, the strength of laser spectroscopy lies in more specialized experiments which often do not lend themselves to such measurements. Other techniques are connnonly used to detect the absorption of light from the laser beam. A coimnon one is to observe fluorescence excited by the laser. The total fluorescence produced is nonnally proportional to the amount of light absorbed. It can be used as a measurement of concentration to detect species present in extremely small amounts. Or a measurement of the fluorescence intensity as the laser frequency is scaimed can give an absorption spectrum. This may allow much higher resolution than is easily obtained with a traditional absorption spectrometer. In other experiments the fluorescence may be dispersed and its spectrum detennined with a traditional spectrometer. In suitable cases this could be the emission from a single electronic-vibrational-rotational level of a molecule and the experimenter can study how the spectrum varies with level. 

In the most general temis, an infrared spectrometer consists of a light source, a dispersmg element, a sample compartment and a detector. Of course, there is tremendous variability depending on the application. 

Light sources can either be broadband, such as a Globar, a Nemst glower, an incandescent wire or mercury arc lamp or they can be tunable, such as a laser or optical parametric oscillator (OPO). In the fomier case, a monocln-omator is needed to achieve spectral resolution. In the case of a tunable light source, the spectral resolution is detemiined by the linewidth of the source itself In either case, the spectral coverage of the light source imposes limits on the vibrational frequencies that can be measured. Of course, limitations on the dispersing element and detector also affect the overall spectral response of the spectrometer. 

Even while Raman spectrometers today incorporate modem teclmology, the fiindamental components remain unchanged. Connnercially, one still has an excitation source, sample illuminating optics, a scattered light collection system, a dispersive element and a detechon system. Each is now briefly discussed. 

Technology developments are revolutionizing the spectroscopic capabilities at THz frequencies. While no one teclmique is ideal for all applications, both CW and pulsed spectrometers operating at or near the fiindamental limits imposed by quantum mechanics are now within reach. Compact, all-solid-state implementations will soon allow such spectrometers to move out of the laboratory and into a wealth of field and remote-sensing applications. From the study of the rotational motions of light molecules to the large-amplitude vibrations of... 

The low MW power levels conuuonly employed in TREPR spectroscopy do not require any precautions to avoid detector overload and, therefore, the fiill time development of the transient magnetization is obtained undiminished by any MW detection deadtime. (3) Standard CW EPR equipment can be used for TREPR requiring only moderate efforts to adapt the MW detection part of the spectrometer for the observation of the transient response to a pulsed light excitation with high time resolution. (4) TREPR spectroscopy proved to be a suitable teclmique for observing a variety of spin coherence phenomena, such as transient nutations [16], quantum beats [17] and nuclear modulations [18], that have been usefi.il to interpret EPR data on light-mduced spm-correlated radical pairs. 

Interference effects, which arise because of the extraordinary uniformity of thickness of the film over the spectrometer sample beam, superimposed on the absorption of incident light by parylene films, can be observed. Experimentally, a sinusoidal undulation of the baseline of the spectmm is seen, particularly in the spectral regions where there is Htde absorption by the sample. These so-called "interference fringe" excursions can amount to some... 

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