Light microscopy dark-field

Bacterial flagella are too thin to be visualized by regular light microscopy (bright field, dark field, or phase contrast microscopy). However, if the microscopes light source is very strong, the flagella scatter sufficient... 

Dark field Visualization technique for ashes produced by microincineration and fluorescence microscopy useful for low-contrast subjects Electron systems imaging EM shadowing Detection, localization, and quantitation of light elements Structural information from ordered arrays of macromolecules... 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

In dark-field microscopy, light from the condenser lens is directed at an angle on to the specimen such that only light which has been refracted or diffracted by the specimen enters the objective lens and forms an image. 

The use of a single polar is compatible with phase contrast microscopy. Crossed polars produce a dark field in which fine fibers will not be seen. If a compensator such as a first order red plate is also used, most of the fine fibers will be seen provided the light source (31) is sufficiently intense. 

Schmidt et. al. [153] investigated the use of dark field microscopy (DFM) to measure fibrous particles. Large areas were examined for statistical reliability. A high correlation was found between DFM and light microscopy for fiber lengths greater than 1 pm. 

It is now also possible to view individual atoms on the surfaces of crystals. One example is by field-ion microscopy, which was developed by Erwin W. Muller." The first images of individual heavy atoms supported on films of light atoms were produced by the use of a dark-field scanning... 

Dark field microscopy (20). in which the relative amplitude of the diffracted orders is changed relative to that of undiffracted light. Usually this involves complete elimination of the undiffracted light, so that the image is featureless apart from the fme detail. 

Bright-Field Illumination A kind of illumination for microscopy in which the illumination of a specimen is arranged so that transmitted light remains in the optical path of the microscope and is used to form the magnified image. This is different from the arrangement in Dark-Field Illumination. 

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