Leukemia cells, removal

To this end, the authors perform a second comet assay. Only limited results, however, are shared PM2 5 from only one city (Baton Rouge) was tested using only one cell line (K562 myeloid leukemia cells), thereby following a broad-to-narrow approach. The results are presented in excerpt 4E. As shown in Eigure 3 (excerpt 4E), cells were exposed to (A) a blank extract, (B) a PM2 5 extract, (C, D, E) a PM2 5 extract mixed with one of three different free radical scavengers, (E) a positive control, and (G, El) a PM2 5 extract mixed with one of two different metal-ion chelators. The free radical scavengers remove free radicals the... 

Another promising immunoconjugate is CMA-676, which is a conjugate of an anti-CD33 MoAb and cali-cheamicin, an anticancer drug shown to be 1000-fold more potent than doxorubicin in animal models. A Phase II trial of 39 acute myeloid leukemia patients resulted in 2 patients with complete remission and 7 patients who showed temporary removal of leukemia cells from the blood. CMA-676 is now in pivotol clinical studies in a number of centers in North America and Europe. ... 

Figure 8. Incorporation of 5-

Conflicting results were obtained in the study of the influence of sialic acid on the flow of ions through the cell membrane Click and Githens (1965) observed a sharp response of the K+ ions to the removal of sialic acid with neuraminidase in L1210 leukemia cells, whereas the transport of Na ions was only slightly inhibited the transport of ions was inhibited regardless of the direction of flow. In Ehrlich ascites cells, however, removal of sialic acid residues with neuraminidase did not alter the content of Na and ions in the cells. Only a very small reduction in the unidirectional fluxes of K ions was observed after neuraminidase treatment. These observations led Weiss and Levinson (1969) to conclude that anionic sites on the cell membrane were not of major importance in regulating the intracellular concentration of Na and K ions or the unidirectional, transmembrane flux of ions. 

Independent Assays for Provings Virus Removal. Retrovimses and vimses can also be present in culture fluids of mammalian cell lines (15,24). Certainly the absence of vims can be difficult to prove. Model vimses, eg, NIH Rausher leukemia vims and NZB Xenotropic vims, were spiked into fluids being purified, and their removal subsequently vaUdated when subjected to the same purification sequence as used for the product. 

Fig. 1. Time dependency of ADP-ribosylation of actin by C2 toxin in rot basophilic leukemia (RBL) cells. Rbl cells were incubated with C2 holotoxin (lOOng/ml C2I + 200ng/ml C2II) for the times indicated. Controls were incubation with the enzyme component C2I (lOOng/ml) alone, or with the binding component C2II (200ng/ml) alone, for 120 min. Another control (-) was not treated with toxin. After incubation, the medium was removed and cells were scraped off in the presence of lysis buffer (2 mM MgCb, 0.1 mM phenylmethylsulfonyl fluoride, 10 ig/ml leupeptin, 25 mM triethanolamine, pH 7.5), sonicated five times for 5sec on ice and centrifuged for 10 min at 1000 g. The supernatants were incubated with 1 [ig/ml C2I and 5nM [ P]NAD for 15 min at 30°C. The proteins were separated by 12.5% SDS-polyacrylamide gel electrophoresis and analyzed by phosphorimaging...

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