Lectin physicochemical properties

Ruff Y et al (2010) Glycodynamers dynamic polymers bearing oligosaccharides residues -generation, structure, physicochemical, component exchange, and lectin binding properties. J Am Chem Soc 132 2573-2584... 

A number of lectins have been purified by affinity chromatography on 0-glycosyl polyacrylamide gels. The physicochemical properties of well-characterized lectins such as Glycine soja, Dolichos biflorus, Phaseolus lunatus. Helix pomatia, and Ricinus communis lectins compared well with those of preparations obtained by alternative chromatographic systems. 

As part of the studies on the structure-function relationships of human tissue alkaline phosphatases, changes in physicochemical properties, activity, affinity for various lectins and blood group antisera, carbohydrate composition,... 

Goldstein, l.J. and Poretz, R.D. (1986) Isolation, physicochemical characterization and carbohydratebinding specificity of lectins. In I.E. Liener, N. Sharon and l.J. Goldstein (Eds.), The Lectins Properties, Functions, and Applications in Biology and Medicine. Academic Press, New York. 

The physicochemical and carbohydrate-binding properties of succinylated wheat-germ agglutin have been compared with those of the unmodified lectin.The dramatic decrease in the apparent number of cell-surface receptors detected using the modified lectin is discussed on the basis of a decrease in the isoelectric point of the lectin and in terms of the acidic properties of the cell surface. 

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