Lane detection

In addition to the practical advantages of replacing side-view mirrors with camera monitor systems, such systems also have strategic importance for the fumre. Sensors on the side of the vehicle will convey essential information for autonomous driving in the fumre. Functions such as blind spot detection, trailer support, lane detection, lane change assist and turn assist are steps on the path to autonomous driving. 

Most of the AHoy 600 outer diameter tube corrosion has occurred in the region of the upper tubesheet near the open lane, ie, an untubed lane across the middle of the steam generator (16,17). The steam carries entrained droplets of water through the open lane to the upper tubesheet region where the droplets dry out and concentrate the chemicals. Long tube inserts have been used to sleeve tubes in this region where wall defects have been detected. 

B) Proteins were resolved by SDS-PAGE, blotted and PG2 polypeptides detected by reaction with anti-PG2 antibodies. Lane 1, 2 pg purified PGl Lane 2, 1 pg purified PG2 Lane 3, 1 pg purified Psubunit. 

FIGURE 10.13 The TLC profiles of labeled peaks isolated from [U- C]ascorbic-acid-modified calf lens protein obtained from Bio-Gel P-2 chromatography. Peaks 2 to 7 were spotted on a preparative silica gel TLC plate and developed with ethanol/ammonia (7 3, v/v). The fluorescence in each lane was detected by irradiation with a Wood s lamp at 360 nm, and the pattern of radioactivity was determined by scanning the plate with AMBIS imaging system. (Reprinted with permission from Cheng, R. et al., Biochim. Biophys. Acta, 1537, 14-26, 2001. Copyright (2001) Elsevier.)... 

The plant sample in lane 6 is also positive for the transgene of interest. Because the band for the effect gene (middle band) is typically fainter than the band for the selectable marker gene (bottom band), it appears that for lane 6, the PCR product amplitication for the effect gene is below the assay detection threshold. Because the selectable marker is clearly present and the PCR amplitication worked, lane 6 can be interpreted as a positive result for the transgene of interest. 

Leinders-Zufall T., Lane A.P., Puche A.C., Ma W., et al. (2000). Ultrasensitive pheromone detection by mammalian vomeronasal neurons. Nature 405, 792-796. 

For any experiments involving the use of phosphospecific antisera, it is crucial to develop the blot (usually a separate one) with an antibody that detects the protein of interest irrespective of its state of phosphorylation. This loading control allows one to assess whether all lanes contain similar amounts of that protein. Ideally, the ratios of the signal strengths for the phospho and total antisera should be calculated from densitometric analysis of the blots. Care must be taken when doing this for proteins that resolve into multiple bands (dependent on their states of phosphorylation,... 

Figure 14 6 Silver-stained SDS-PAGE gel of PatA binding proteins. Lane 1, sample 1 nonspecific proteins captured by the streptavidin-agarose resin Lane 2, sample 2 proteins affinity captured by the presence of B-Pat A Lane 3, sample 3 affinity capture of target proteins was blocked by prior addition of free PatA before incubation with B-PatA. The two arrows point to two proteins specifically detected in sample 2 versus sample 1, which were also lost due to competition in sample 3, with apparent molecular weights of 38 and 48 kDa.

Fig. 8.1 Western blot analysis of transgenic lines showing the expression of an assembled monoclonal antibody in transgenic chloroplasts. Lane 1 Extract from a chloroplast transgenic line, Lane 2 Extract from an untransformed plant. Lane 3 Positive control (human IgA). The gel was run under non-reducing conditions. The antibody was detected with an AP-conjugated goat anti-human kappa antibody.

Fig. 8.11 (A and B) Expression and purification of insulin-polymer fusion protein detected in copper (A) and Coomassie (B) stained gels. The same gel was first stained with copper, destained and restained with Coomassie R-250. Lane 1 Prestained marker Lane 2 Purified extract of polymer-insulin fusion protein from the chloroplast vector pSBL-OC-40Pris Lane 3 Reverse orientation of fusion protein from pSBL-OC-40Pris Lane 4 Purified extract of polymer-insulin fusion protein from the chloroplast vector pLD-OC-40Pris ...

Figure 13.15 Detection of a specific sequence of DNA by hybridization to a 32P-labelled cDNA probe. DNA is transferred to nitrocellulose and incubated with the probe. After washing, specific binding is visualized by autoradiography. The DNA sequence detected by the probe is present in lanes 2, 3 and 5 but not 1 and 4.

Fig. 2.139. Upper lane densitograms of colour pigments of Trichoderma harzianum. Alugram RP-I8W/UV254 plates. Gradient water-acetone (10 90, v/v) for 3cm water-acetone (45 55, v/v) for 13cm. Detection wavelength 400 nm. Lower lane RP-HPLC chromatogram of colour pigments of Trichoderma harzianum. Reprinted with permission from G. Csiktusnadi Kiss et al. [312].

Fig. 3.130. HPLC chromatograms of the test mixture detected by DAD (270 nm, upper lane) by APCI-MS-TIC (middle) and by ESI-MS-TIC (lower lane). Peak identification l=benzene sulphonic acid sodium salt 2=2-naphtalene sulphonic acid sodium salt 3=2-anthraquinone sulphonic acid sodium salt 4 = sulphorhodamine D sodium salt 5=crocein orange G 6=eriochrome black T 7=2,6-anthraquinone disulphonic acid disodium salt 8 = 1,5-naphtalene disulphonic acid disodium salt 9 = azophloxine 10 = 1,2-benzene disulphonic acid dipotassium salt. Reprinted with permission from G. Socher et al. [178].

A schematic representation of a CE system is presented in Figure 9.1. In this diagram, the CE components have obvious counterparts to those found in slab gel electrophoresis. Instead of buffer tanks there are two small buffer reservoirs, and the capillary takes the place of the gel (or more accurately, a gel lane). The capillary is immersed in the electrolyte-filled reservoirs, which also make contact with the electrodes connected to a high-voltage power supply. A new feature to the conventional gel electrophoresis format is the presence of an online detection system. 

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