Laminins composition

Uchida, M., Oyane, A., Kim, H.-M., Kokubo, T. and Ito, A. (2004) Biomimetic coating of laminin-apatite composite on titanium metal and its excellent cell-adhesive properties. Advanced Materials, 16, 1071-1074. 

Oyane, A., Uchida, M. and Ito, A. (2005) Laminin-apatite composite coating to enhance cell adhesion to ethylene-vinyl alcohol copolymer. Journal of Biomedical Materials Research, 72A, 168-174. 

Collagen IV has been found to bind to both laminin and heparan sulfate proteoglycan, whereas laminin binds both the nidogen and heparan sulfate proteoglycan. These observations suggest that multiple interactions of a specific nature could be important in determining both the composition and the deposition of basement membranes. 

Eukaryotic cells have an internal scaffolding called the cytoskeleton or cytomatrix that maintains their cellular morphology and enables them to migrate, undergo shape changes, and transport vesicles. Microfilaments, made of actin, intermediate filaments, which are composed of laminin and other proteins, and microtubules, formed from the protein tubulin, along with many different accessory proteins, comprise the cytoskeleton. Both the microfilaments and the microtubules can assemble and disassemble rapidly in the cell, whereas disassembly of intermediate filaments may require their destruction. Although much is known about the molecular composition of the cytoskeleton, the molecular events involved in most cell movements are still unknown. 

Chitosan-collagen composite Isolated aortic valve endothelial cell (VEC) cultures and preferential adhesion to fibronectin, collagen types IV and I over laminin and osteo-pontin. Chitosan-collagen type IV films act as protein precoatings The composite showed improved VEC growth and morphology in comparison to chitosan alone Certain alteration in the properties of chitosan can improve amenability to valve tissue engineering applications Cuy et al. [126]... 

Integrins Transmembrane a)S-heterodimer a 165 kDa j , 90-120 kDa Plasmalemma Binding to laminins, fibronectin, collagen specificity depends on a)S composition Maintenance of cell to basement membrane and extracellular matrix binding... 

M. Uchida, A. Ito, K.S. Furukawa, K. Nakamura, Y. Onimura, A. Oyane, T. Tateishi, et al.. Reduced platelet adhesion to titanium metal coated with apatite, albumin-apatite composite or laminin-apatite composite. Biomaterials 26 (34) (2005) 6924-6931. 

ZnO and MgO nanoparticles have been shown to increase bone cell functions and decrease infection (Liu et al., 2015 Weng and Webster, 2012). Nano zinc oxide (ZnO) also can induce osteogenic properties from stem cells (Liu et al., 2015). Composites incorporating ZnO nanoparticles with a diameter near 60 nm can form a scaffold for tissue regeneration. The size of laminin, collagen, and fibronectin, which are all major components of the natural ECM, is on the same order of magnitude as a ZnO nanoparticle. Moreover, the piezoelectric and antibacterial properties of ZnO particles make it a good choice for orthopedic implant applications (Sell and Webster, 2(X)8). 

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