Laminar flow hoods

Carolina in 1981. Each sample (1.4 ml of methanolic sample) was placed into a 3-cm petri dish and the solvent evaporated under a laminar flow hood at room temperature. Seventy seeds (0.035 g) were then placed into the petri dishes and 1.4 ml of sterilized (0.2 pm-filter) 15 mM Mes [2-(N-morpholino)ethanesulfonic acid Sigma Chemical Co.] buffer adjusted to pH 5.5 was added. The dishes were kept in the dark at 25°C2for 84 hr, exposed to 12-hr fluorescent light (250 p einsteins/m /sec), and then placed back in the dark for an additional 4 days (17). Percent germination, root and hypocotyl lengths were then determined. 

Material required Air-dried (27-30°C) aerial parts of S. deppei to prepare aqueous leachate, Seeds of tomato (Lycopersicon esculentum Mill. cv. Rio Grande), Osmometer, Growth Chamber Laminar flow hood. 

Bioassays are performed under sterile conditions in a laminar flow hood. Tomato seeds are previously washed and disinfected with 1% sodium hypochlorite. Seeds are germinated in the Petri dishes containing the S. deppei aqueous leachate. For control, seeds are germinated in 1% agar. Twelve seeds are placed on each Petri dish and kept in the dark at 27°C in a growth chamber. For enzyme activities, 40-50 Petri dishes are used per treatment. Primary roots (radicles) are excised after 72 h, frozen in liquid nitrogen and kept at -70 °C until use. For root growth response, experiments... 

The equipment needed is determined by the type and extent of the services chosen to provide. Hospitals already utilize laminar flow hoods for aseptic compounding of sterile solutions. The same hoods can be used to compound other sterile products such as eye drops. A balance, preferably electronic, is essential. Ointment slabs (pill tiles), along with spatulas of different types and materials, should be on hand. A few mortars and pestles (both of glass, ceramic, and/or plastic) should be obtained and some glassware. It may not be necessary to buy a roomful of equipment, but one should purchase what is needed to start the service, and build it up as the service grows and expands to different arenas. 

Assay at 5, 10, and 15 min intervals. Perform the entire operation in a laminar-flow hood. At the conclusion of each challenge time interval, pass the contents of each tube through a separate membrane filter unit. Wash each membrane with 3 X 100 ml portions of neutralizer solution. Remove each membrane from its filter unit and place face up on the surface of appropriate poured agar plate. Positive controls must be tested last. 

Locations of laminar flow hoods, biosafety hoods, and exhaust hoods are noted. 

A floor plan of the facility that includes locations (or identification of locations) of equipment, including laminar flow hoods, sterilizers, lyo-philizers, filling heads and equipment segregated by barrier or isolation systems, is included in (reference attachment no.). The filling equipment is located in the level 1 unidirectional laminar flow hood areas. 

All finished products are tested for sterility in a controlled-access clean room environment in a laminar flow hood, which provides no greater level of a microbial challenge than that encountered in the fill environment. The testing environment consists of a gowning room and sterility test suite that meets the same air quality standards as the hlling suite. 

C.O.R = centre of the room. LFH = under laminar flow hood. LAF Laminar flow... 

Perform the entire operation in a laminar flow hood with aseptic technique. 

Under a laminar flow hood, aseptically place 20 stoppers of the size and mold number to be tested into a sterile jar containing 50 ml of 0.1% Tween solution such that all stoppers are completely immersed (100 ml 0.1% Tween for 28-mm stoppers). 

After heat shocking, remove the rinse samples and place in cool water. After cooling, shake container on mechanical shaker at slow setting for 30 minutes. Sonicate for 2 minutes just prior to testing. Transfer the samples to a laminar flow hood. 

Gently mix the stoppers in the container to ensure that all surfaces come in contact with the water and sonicate for 30 minutes. Return the container to the laminar flow hood. Open the container and, using an automatic pipettor fitted with sterile, nonpyrogenic tips, pipette 0.1 ml of the water into each of two sterile pyrogen-free test tubes. 

Working under a laminar flow hood, add sterile pyrogen-free WFI to the vials to be tested. The following amounts are added ... 

Sterile forceps Vacuum pump Laminar flow hood Water bath set at 45 to 55°C Incubator set at 30 to 35°C Mechanical shaker Sterile surgical gloves Self-contained anaerobe jar Bunsen burner Sterile, pyrogen-free WET... 

Inoculation Inoculation is the seeding of a culture vessel with the microbial material (inoculum). The inoculum is introduced with a metal wire or loop which is rapidly sterilized just before its use by heating it in a flame. Transfers of liquid culture are often made by using a sterilized pipette. The inoculation is usually done in a laminar flow hood to minimize the risk of contamination. It is important to know proper pipetting techniques for inoculating or sampling during cultivation. 

Lyophilizers Laminar flow hoods Ductwork, HVAC, and air filters Walls, floors, and ceilings... 

Laminar flow hoods are another gray area with regard to product contact. There is the potential for product contact and thus contamination since the product is opened in these areas and air is flowing over open product for a finite period. The normal activity, however, should not result in product contamination unless the surfaces of the laminar flow hood are grossly contaminated, and visual inspection should certainly mitigate against such extensive and obvious contamination. Again, it may be possible to develop... 

An electrical resistance heater with more turns at the tube ends (to compensate for heat losses) surrounds each tube. There is a vertical laminar flow hood over the loading area to minimize particle contamination of the wafers being loaded. As we can see, there are temperature controls for the furnace tubes, and a power module to provide the electrical power. When operated as a LPCVD system, a unit including both the gas flow and vacuum systems is positioned on the right side. Such a unit is shown in Figure 8. Here we can see the vacuum pumps on the left, and the mass flow controllers on the right. The vacuum pump oil recirculation systems are shown in the slide out drawers. As can be seen in Figure 9, this system, as well as most current similar systems, operate under computer control. 

In addition to the design goal of reducing the processing cost per wafer, this system has been developed to reduce the particulate contamination to a minimum. To accomplish this, the entire system is enclosed within a laminar flow hood, so that the wafers are handled in a class 10 environment. Secondly, wafers are loaded and unloaded by an automated robot, so that human intervention is not necessary to handle the two susceptors. Both of these features are illustrated in Figure 15. 

This procedure is best performed in a laminar flow hood but with care can easily be done on the laboratory bench. Although Sendai is a rodent virus, it is always a sensible precaution for laboratory personnel to wear gloves, gowns and masks when handling the virus or infected animals. 

Sterilgard III Advance tissue culture laminar flow hood (Baker Co., ME)... 

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