Lactobacillus acidophilus extract

Affinity Separation - Treatment of dCTP with carbonylbis(imidazole) followed by N-trifluoroacetyl-6-aminohexyl phosphate affords (191), which was deacetylated and coupled via its aminoalkyl group to CNBr-Sepharose, affording a P4 (dC) affinity column (192).326 This column gave a one-step, 19,000-fold purification to homogeneity of deoxycytidine kinase from a crude ammonium sulphate fraction of Lactobacillus acidophilus R-26 extract, with 60 % recovery, a striking instance of the power of affinity chromatography. Elution from the column was performed with dCTP, which is a powerful end-product inhibitor for the enzyme and serves to stabilize it. 

Lin, T. Y., Lin, C. W., Wang, Y. J. (2002). Linoleic acid isomerase activity in enzyme extracts from Lactobacillus acidophilus and Propionibacterium ffeudenreichii ssp. shermanii. Journal of Food Science, 67,1502-1505. 

Extracts of certain bacteria requiring 2-deoxyribonucleosides, namely, Lactobacillus helveticus, Lactobacillus delbriickii, and Thermobacterium acidophilus R 26, catalyze the transfer of the 2-deoxy-D-ribosyl group from one purine or pyrimidine to another. " 2-Deoxyribosyl -uracil, thy-... 

Living biofilms are not a strict prerequisite for adhesion prevention. Excreted compounds such as EPS or biosurfactants alone are often inhibiting. Purified biosurfactants from Lactobacillus casei, L. fermentum, and L. acidophilus were able to reduce the number of pathogenic Enterococcus faecalis to biosurfactant-coated glass by up to 77 % compared to uncoated controls [20]. Based on the method used for extracting and purifying biosurfactants, one can assume that bacterial EPS were indeed these... 

Enzyme preparations from Lactobacillus delbrueckii and Thermobacterium acidophilus R 26 catalyze similar transfer reactions, as do extracts of E. coli. ... 

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