Lactic acid .dehydrogenase

Revised spectrophotometric methods for the determination of glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase and lactic acid dehydrogenase. Am. J. Clin. Path. (1960), 34, 381-398. 

Hensel, R., Mayr, R., Stetter, K. 0. and Kandler, O. 1977. Comparative studies of lactic acid dehydrogenase in lactic acid bacteria. I. Purification and kinetics of the allosteric L-lactic acid dehydrogenase from Lactobacillus casei spp. casei and, Lactobacillus curvatus. Arch. Microbiol 112, 81-93. 

Some examples follow that illustrate the remarkable specificity of this kind of redox system. One of the last steps in the metabolic breakdown of glucose (glycolysis Section 20-10A) is the reduction of 2-oxopropanoic (pyruvic) acid to L-2-hydroxypropanoic (lactic) acid. The reverse process is oxidation of l-lactic acid. The enzyme lactic acid dehydrogenase catalyses this reaction, and it functions only with the L-enantiomer of lactic acid ... 

Figure 5. Lactic acid dehydrogenase schematic. This schematic illustrates the value of LDH isozyme patterns for the identification of menstrual blood and seminal material.

NS), respectively, cytochrome oxidase decreased 9%(NS). Treatment-related effects in wmn muscle citrate synthetase decreased 19% (NS), cytochrome oxidase increased 23% (NS), muscle lactate and lactic acid dehydrogenase affected. Subjects reported no adverse health effects after exposure. 

Singh, S. D., and Somani, I. K., Serum lactic acid dehydrogenase levels in progressive muscular dystrophy in children. Indian Fractit. 16, 433 (1963). 

Acute rhabdomyolysis with fever, muscle cramps, rises in creatine phosphokinase and lactic acid dehydrogenase activity, phlebitis at the injection site, and transitory leukopenia and thrombocytopenia have been reported (8). 

Lactic acid dehydrogenase from a crude bovine heat extract [11]... 

The cross-axis CPC (coil planet centrifuge), with column holders at the off-center position on the rotary shaft, enables retention of the stationary phase of aqueous-aqueous polymer-phase systems such as PEG 1000-potassium phosphate and PEG 8000-dex-tran T500 [3,4], Since the last decade, various types of cross-axis CPC (types XL, XLL, XLLL, and L) have been developed for performing CCC with highly viscous aqueous polymer-phase systems. The separation and purification of protein samples, including lactic acid dehydrogenase [5], recombinant enzymes, profiUn-actin complex, and so on, were achieved using these cross-axis CPCs [6]. 

The performance of the XL cross-axis CPC, equipped with a pair of columns with a 165-mL capacity, was evaluated for purification of lactic acid dehydrogenase (LDH) from a crude bovine heart filtrate. Successful separation of the LDH fraction was achieved with 16% (w/w) PEG 1000-12.5%(w/w) potassium phosphate at pH 7.3. The separation was performed at 500 rpm at a flow rate of 1.0 mL/min using the potassium phosphate-rich lower phase as a mobile phase. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the LDH fractions showed no detectable contamination by other proteins. The enzymatic activity was also preserved in these fractions. 

W8. Wieland, T. H., and Pfleiderer, G., Chemical differences between multiple forms of lactic acid dehydrogenases. Ann. N.Y. Acad. Sci. 94, 691-700 (1961). 

Gault, M. H., Cohen, M. W., Kahana, L. M., Leelin, F. T., Meakins, ]. F., and Aronovitch, M., Serum enzymes in patients with carcinoma of the lung. Lactic acid dehydrogenase, phosphohexose isomeiase, alkaline phosphatase and glutamic ox-alacetic transaminase. Can. Med. Assoc. J. 96, 87-94 (1967). 

The NAD -NADH coenzyme system is involved in a large number of biological oxidation-reductions. Another reaction similar to the ethanol-acetaldehyde conversion is the oxidation of lactic acid to pyruvic acid by NAD and the enzyme lactic acid dehydrogenase ... 

Phisphumvidhi, P., and Langer, B. W., Jr. (1969). Malarial parasite metabolism The lactic acid dehydrogenase of Plasmodium berghei. Exp. Parasitol. 24, 37-41. 

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