Aqueous two-phase polymer systems

The phenomenon of aqueous two-phase polymer systems has been known since the 19th century. However, the utilization of aqueous two-phase systems as a separation method for biological materials was explored much later by Albertsson in the mid-1950s. Albertsson performed an extensive investigation on the ability of different water-soluble polymers to form aqueous two-phase systems and how biological macromolecules and cell particles partitioned in these phase systems [27]. 

PIE Pietraszka, N., Galaev, I.Yu., Kumar, A., Brzozowski, Z.K., and Mattiasson, B., New polymers forming aqueous two-phase polymer systems, Biotechnol. Progr., 16, 408, 2000. 

KAN Kang, C.H. and Sandler, S.I., Effects of polydispersivity on the phase behavior of aqueous two-phase polymer systems. Macromolecules, 21, 3088, 1988. 

HA2 Haynes, C.A., Blanch, H.W., and Praunsitz, J.M., Separation of protein mixtures by extraction thermodynamic properties of aqueous two-phase polymer systems containing salts and proteins, Fluid Phase Equil, 53, 463, 1989. 

ALM Al-Muallem, H.A., Wazeer, M.I.M., and Ali, Sk.A., Synthesis and solution properties of a new ionic polymer and its behavior in aqueous two-phase polymer systems. Polymer, 43, 1041, 2002. 

Aqueous two-phase polymer systems formed by SPs for use in bioseparation... 

Hooker, B.S. and Lee, J.M. (1990) Cultivation of plant cells in aqueous two-phase polymer systems. Plant Cell Rep, 8, 546. 

The introduction of partition in aqueous two-phase polymer systems by Albertsson (1971) contributed, in the recent past, to the isolation of plant membranes and organelles. This method, based upon the membrane surface properties in particular, resulted in the separation of plasma membranes from E. R., Golgi membrane or plastid envelopes. In the present study, we adapted this procedure in order to isolate and characterize a plasma membrane fraction from fungal material. 

Brunette, D. M., and Till, J. E., 1971, A rapid method for the isolation of L-cell surface membranes using an aqueous two-phase polymer system, /. Membrane Biol. 5 215. 

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