Kluyveromyces marxianus

A series of 2-(4-chlorophenoxy)-3-oxoalkanoate were reduced by baker s yeast, and Kluyveromyces marxianus. Yeast reduction of ethyl 2-(4-chlorophenoxy)-3-oxo-3-phenylpropanoate (R=Ph) afforded enantiomerically pure ethyl 2R, 3S)-2-(4-chlorophenoxy)-3-hydroxy-3-phenylpropanoate out of the four possible stereoisomers in >99% de [29h]. Although baker s yeast reduction of butanoate (R = CFl3) was not selective (92% de), the use of K. marxianus afforded (2R, 3S)-isomer selectively [29i]. The products are intermediates for potential peroxisome prolifera-tor-activated receptor isoform a-agonists (Figure 8.39a). 

Endo-polygalacturonase of the yeast Kluyveromyces marxianus is constitutive, highly active on native pectin and is the main extracellular protein... 

Kluyveromyces marxianus CCT 3172 was isolated from cocoa fermentations in Bahia, Brazil and has been deposited in the culture collection of the Funda ao Tropical de Pesquisas e Tecnologia "Andre Tosello" (CCT), Campinas, Sao Paulo, Brazil. 

A commercial pectinase, immobilised on appropriately functionalised y-alumina spheres, was loaded in a packed bed reactor and employed to depolymerise the pectin contained in a model solution and in the apple juice. The activity of the immobilized enzyme was tested in several batch reactions and compared with the one of the free enzyme. A successful apple juice depectinisation was obtained using the pectinase immobilised system. In addition, an endopolygalacturonase from Kluyveromyces marxianus, previously purified in a single-step process with coreshell microspheres specifically prepared, was immobilised on the same active support and the efficiency of the resulting catalyst was tested. 

The endopolygalacturonase obtained from a Kluyveromyces marxianus culture broth was purified through the addition of specifically designed core-shell microspheres consisting of an inner polystyrene core and an outer shell constituted by a poly(methacrylic acid-co-ethylacrylate) statistical copolymer. These microspheres were previously found very effective in purifying the pectinlyase within a commercial pectinase sample [15]. 

Several scouting experiments were performed to find the best pH conditions. Figure 3 reports the ratio between the PG specific activity measured after the purification procedure (ASf) and the initial PG specific activity (ASi). At pH 3.5, the microspheres are able to remove from the broth the major part of the protein without PG activity, thus providing a four time increase of the enzyme specific activity. The purified PG from Kluyveromyces marxianus was immobilised following the above procedure. Batch reactions in the packed bed reactor were done to evaluate the biocatalyst stability. After an initial loss, due to enzyme release, the residual PG activity reaches a plateau value corresponding to about 40% of the initial activity. Probably, some broth component interfered during the immobilisation reaction weakening the protein-carrier interactions. 

Recovery of inulinase from Kluyveromyces marxianus fermentation broth [16]... 

Artolozaga, M. J., Jonas, R., Schneider, A. L., Furlan, S. A., and Carvalho-Jones, M. F., One step partial purification of fi-D-ga lactosidase from Kluyveromyces marxianus CDB 002 using STREAMLINE-DEAE, Bioseparation, 7, 137, 1998. 

Meehan C, Banat IM, McMullan G et al (2000) Decolorization of remazol black-B using a thermotolerant yeast, Kluyveromyces marxianus IMB3. Environ Int 26 75-79... 

The gene for calf chymosin has been cloned in Kluyveromyces marxianus var. lactis, Aspergillus niger and E. coli. Microbial (cloned) chymosins have... 

Kefir Kefir grains - thermophilic lactobacilli and Kluyveromyces marxianus... 

Kefir and Koumiss contain about 1 and 6% ethanol, respectively, which is produced by lactose-fermenting yeasts, usually Kluyveromyces marxianus. The ethanol modifies the flavour of the products and the C02 produced in the fermentation affects both their flavour and texture. Koumiss, which is produced traditionally from mares milk, mainly in Russia and surrounding areas of Asia, is not in fact coagulated. 

Hansenula petersonii Kluyveromyces bulgaricus Kluyveromyces marxianus Lipomyces kononenkoae Lipomyces starkeyi Lipomyces tetrasporus Pichia guilliermondii... 

Snyder and Phaff74 classified their enzyme from Kluyveromyces marxianus as inulinase (EC 3.2.1.7) rather than /3-D-fructofuranosidase (EC 3.2.1.26). Their inulinase hydrolyzed inulin from the D-fruc-tosyl end of the polymer, by an endwise action.81,82 Release of the en-... 

Detailed studies have been made on the /3-D-glucosidases of the following (t) Rhodotorula minuta (misnamed Saccharomyces cerevi-stae),32-335,338 (it) Kluyveromyces (Saccharomyces) lactis,337-344 (tit) Kluyveromyces (fragilis) marxianus,348,348 (iv) Kluyveromyces dobz-hanskii,348 (o) a hybrid of Kluyveromyces marxianus and Kluyveromyces dobzhanskii,348-351 (t>t) a yeast of unknown identity (see Ref. 4) called Saccharomyces cerevisiae, 36 3M-3 8 (mi) Pichia (Candida) guilliermondii,331 and (viii) Debaryomyces polymorphus (Pichia poly-morpha).358 Table X lists some characteristics of these /3-D-glucosi-... 

Goncalves, J. A. and Castillo, F. J. 1982. Partial purification and characterization of (3-D-galactosidase from Kluyveromyces marxianus. J. Dairy Sci. 65, 2088-2094. 

White/cream/brownish wrinkled/striated to rough Oval and short cylindrical Kluyveromyces marxianus... 

Ferreira et al. [103] Lactose Yeast Kluyveromyces marxianus fermentation / -Galactosidase and glucose oxidase Oxygen electrode ... 

Whey Kluyveromyces marxianus var. marxianus P-lg YLLF ACE-inhibitory Belem et al. (1999)... 

Debaryomyces polymorphus Kluyveromyces marxianus var. bulgaricus Kluyveromyces marxianus var. marxianus Saccharomyces cerevisiae Schwanniomyces occidentalis var. occidentalis Torulaspora delbrttckii 96 Barthomeuf etal., 1991... 

Margaritis, A. and Bajpai, P., Ethanol production from Jerusalem artichoke tubers (Helianthus tuberosus) using Kluyveromyces marxianus and Saecharomyces rosei, Biotechnol. Bioeng., 24, 941-953, 1982a. 

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