Kluyveromyces lactis, 3-galactosidase

Work that may prove of immense industrial significance is the isolation from Kluyveromyces lactis, by Dickson and Markin,394 of the gene for /3-D-galactosidase by use of recombinant-DNA techniques. The DNA from the yeast was partly digested with endonuclease, and the product was joined to a plasmid DNA by means of DNA ligase. The ligated DNA was then successfully used to transform a lactose-nega-... [Pg.396]

Galactosidases of Aspergillus oryzae, Penicillium multicolor, Kluyveromyces lactis, and Escherichia coli were reported to form, 1-6-linkages [21]. Employment of /3-galactosidase of Xantomonas manihotis and recombinant Bacillus circulans (bga C gene) also gave considerable amounts of, 1-6-linked disaccharides [30],... [Pg.1395]

Only just over 10% of yeast species utilize lactose (see Table III), and most of these species also utilize D-galactose. /3-D-Galactosidase (EC 3.2.1.23) activities have been studied chiefly for two species, namely, Kluyveromyces /ragtits221,223,313,577-587 and Kluyveromyces lactis.545,588... [Pg.208]

Athes, V., Lange, R. and Combes, D., 1998, Influence of polyols on the structural properties of Kluyveromyces lactis beta-galactosidase under high hydrostatic pressure. European Journal of Biochemistry 255, 206-212. [Pg.389]

Baur X, Sander I, Jansen A et al. (1994) Are amylases in bakery products and flour potential food aUeigens Schweiz Med Wochenschr 124(20) 846-851 Becerra M, Cerdan E, Gonzalez Siso MI (1997) Heterologous Kluyveromyces lactis beta-galactosidase production and release by Saccharomyces cerevisiae osmotic-remedial thermosensitive autolytic mutants. Biochim Biophys Acta 1335(3) 235-241 Bertoldo C, Antranikian G (2002) Starch-hydrolyzing enzymes from thermophilic archaea and bacteria. Curr Opin Chem Biol 6 151-160... [Pg.90]

Champluvier B, Francart B, Rouxhet PG. (1989b). Co-immobilization by adhesion of (1-galactosidase in nonviable cells of Kluyveromyces lactis with Klebsiella oxytoca-. Conversion of lactose into 2,3-butanediol. Biotechnol Bioeng, 34, 844—853. [Pg.281]

Rodriguez-Colinas B, de Abreu MA, Femandez-Arrojo L, de Beer R, Poveda A,Jimenez-Barbero J, et al. Production of galacto-ohgosaccharides by the beta-galactosidase from Kluyveromyces lactis comparative analysis of permeabUized cells versus soluble enzyme.] Agric Food Chem 2011 59(19) 10477-84. [Pg.673]

Kim C.S., Ji E.S. Oh D.K. (2003). Expression and characterization of Kluyveromyces lactis beta-galactosidase in Escherichia coli. Biotechnol. Lett., 20,1769-1774. [Pg.371]

P-D-Galactosidase Aspergillus niger, A. oryzae, Kluyveromyces fragilis, K. lactis 1,2,4,18... [Pg.147]

Kluyveromyces Ascospore producing yeast multiplies by binary fission Production of fS-galactosidase andlaccase K.fraglis K. lactis K. marxianus... [Pg.61]

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