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Galactosidase Aspergillus niger

P-D-Galactosidase Aspergillus niger, A. oryzae, Kluyveromyces fragilis, K. lactis 1,2,4,18... [Pg.147]

The authors wish to thank Maijo Searle-van Leeuwen for purification of the P-galactosidase from Aspergillus niger and Novo Nordisk Ferment Ltd Dittingen (Switzerland) for assistance in the purification of the RG-hydrolase and Novo Nordisk A/S Bagsvaerd (Denmark) for the gift of the crude recombinant RG-lyase. [Pg.273]

Enzyme labels are usually coupled to secondary antibodies or to (strept)avidin. The latter is used for detection of biotinylated primary or secondary antibodies in ABC methods (see Sect. 6.2.1). Enzyme labels routinely used in immunohisto-chemistry are horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase (AP). Glucose oxidase from Aspergillus niger and E. coli (3-galactosidase are only rarely applied. [Pg.15]

Enzymatic markers used in immunohistochemistry Horseradish peroxidase (HRP) and calf intestinal or E.coli alkaline phosphatase (AP). Glucose oxidase from Aspergillus niger and E.coli /3-galactosidase are only rarely applied. [Pg.145]

Application and Principle Use this procedure to determine a-galactosidase activity in enzyme preparations derived from Aspergillus niger var. The assay is based on a 15-min hydrolysis of p-nitrophenyl-a-D-galactopyranoside followed by spec-trophotometric measurement of the liberated p-nitrophenol. [Pg.905]

Lactose A thermostable p-galactosidase encoded by lacA from Aspergillus niger was expressed from aADHl promoter in p-galactosidase activity, 40% was secreted to the cellular medium, and the recombinant strain grew on whey permeate with a specific growth rate of 0.43 h 75... [Pg.198]

Shin JH, Lee GM, Kim JH (1994) Comparison of cell disruption methods for determining (3-galactosidase activity expressed in animal cells. Biotechnol Tech 8(6) 425 30 SMnmyo A, Davis IK, Nomoto F et al. (1978) Catabolite repression of hydrolases in Aspergillus niger. J Appl Microbiol Biotechnol 5(l) 59-68... [Pg.103]

Flaschel, E., Raetz, E., and Renken, A. (1982) The kinetics of lactose hydrolysis for the p-galactosidase from Aspergillus niger. Biotechnol. Bioeng.,... [Pg.87]

The isolated fractions of AcGGM were separately incubated with the Aspergillus niger endo-l,4-p-mannanase of family 5 (29) and the family 27 a-galactosidase (30) at enzyme loadings of 5 or 10 nkat/mg substrate incubated at 40 C for 48 hours at pH 5.0 and 4.8, respectively (2). The produced saccharides were analyzed either by determination of reducing sugars or by HPAEC (2). [Pg.69]

Figure 5. Liberation of arabinose and galactose from arabino-l,3/6-galactan by three side-chain-acting enzymes a-arabinosidase, fi-galactosidase and 1,6-galactanase from Aspergillus niger (18,31),... Figure 5. Liberation of arabinose and galactose from arabino-l,3/6-galactan by three side-chain-acting enzymes a-arabinosidase, fi-galactosidase and 1,6-galactanase from Aspergillus niger (18,31),...
Rarkas, T., Stalbrand, H., Tjemeld, R. (1996). Partitioning of beta-mannanase and alpha-galactosidase from Aspergillus niger in ucon/reppal aqueous two-phase systems and using temperature-induced phase separation. Bioseparation, 6, 147-157. [Pg.34]

The jS-D-galactosidase from Aspergillus niger immobilized by adsorption onto either alumina or titania-treated stainless steel retained essentially all of its activity when stored under water at 23 °C for long periods little activity was lost when cheese whey was treated with the immobilized enzyme at 55 °C over several weeks. The catalytic activity of the immobilized enzyme (maximum activity at pH 3 and 60 °C) was unaffected by frequent sanitizations. Nickel-nickel oxide sinters were also used to immobilize this j8-D-galactosidase. Gluco-amylase was also successfully immobilized on these matrices. [Pg.460]

O.P. Bahl and K.M.L. Agrawal, a-Galactosidase, 3-galactosidase, and P-N-acetylglucosami-nidase from Aspergillus niger. Methods Enzymol, 1972, 28, 728-734. [Pg.1618]


See other pages where Galactosidase Aspergillus niger is mentioned: [Pg.147]    [Pg.812]    [Pg.147]    [Pg.812]    [Pg.353]    [Pg.264]    [Pg.266]    [Pg.266]    [Pg.761]    [Pg.202]    [Pg.167]    [Pg.208]    [Pg.229]    [Pg.230]    [Pg.164]    [Pg.1535]    [Pg.148]    [Pg.896]    [Pg.19]    [Pg.121]    [Pg.240]    [Pg.291]    [Pg.260]    [Pg.113]    [Pg.407]    [Pg.206]    [Pg.7]    [Pg.25]    [Pg.60]    [Pg.443]    [Pg.1286]    [Pg.310]    [Pg.511]    [Pg.417]    [Pg.372]    [Pg.73]    [Pg.342]   
See also in sourсe #XX -- [ Pg.229 ]




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Galactosidase

Galactosidases Aspergillus niger

Galactosidasic

Niger

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