Kloeckera brevis

O-Phosphono-D-mannans have been isolated from several strains of yeast. Stewart and Ballou have observed a great variation in the phosphorus content of diflFerent mannans, the highest being in Kloeckera brevis mannan, which has one phosphorus atom for every nine D-mannose residues. These phosphonomannans appeared to have a structure similar to that of the mannan already described, except for the presence of phosphate groups. ... 

The species most commonly employed for thiamine determination are some Lactobacilli such as L.fermentum and L. viridescens (ATCC 12706). The latter is the microorganism most widely used to measure thiamine concentrations. It requires the intact thiamine molecule for growth. Other organisms such as Phycomyces blakeslekanus, Kloeckera brevis (ATCC 9774), Ochromonas danica and Neurospora crassa are available, but they are less useful. 

Strictly specific for thiamine are the yeast Kloeckera brevis and the bacteria Lactobacillus fermentum 36 and Lactobacillus viridescens. The first will measure 0.2-2.0 m g of thiamine, the two latter 5-40 m/ g. They all also respond to thiamine pyrophosphate, but to a different degree therefore the material under assay should be hydrolysed by a phosphatase preparation prior to assay. 

Thieme, T. R. and Ballou, C. E. (1972) Subunit structure of the phosphomannan from Kloeckera brevis yeast cell wall. Biochemistry, 11,1115-1120. 

These methods were the first developed for biotin analysis and are still accepted as official methods because of their high sensitivity (nanogram level). The species most commonly employed for biotin determination are some Lactobacilli such as L. arabinosus (ATCC 2112), L. plantarum (ATCC 8014), and L casei (ATCC 7469) Ochromonas danica, Neurospora crassa, and Saccharomyces cerevisae (ATCC 4228) are also employed. Kloeckera brevis (ATCC 9774) is useful for a radiometric-microbiological determination based on the measurement of " C02 formed by the test microorganism from " C-labeled L-methionine. 

For normal growth with different sources of carbon, many yeasts require myo-inositol in small quantities, as it is important in lipid metabolism.122 Accordingly, strains of Kloeckera apiculata (brevis),655,656 Saccharomyces uvarurn (carlsbergensis),657 658 Schizo-... 

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