Kinetics of Allosteric Proteins

The substrate functions as a positive modulator i.e., there is positive cooperativity between the substrate binding sites so that binding of the substrate at one 

Relationship between the initial velocity (v) and the substrate concentration [S] for an allosteric enzyme that shows a homotropic effect. The substrate functions as a positive modulator. The profile is sigmoidal, and during the steep part of the profile, small changes in [S] can cause large changes in v. Ko.i represents the substrate concentration corresponding to half-maximal velocity. 

Owing to the above effect, the shape of the curve is sigmoidal. 

The value of the substrate concentration corresponding to half-maximal velocity is designated as A o.5 and not since the allosteric kinetics do not follow the hyperbolic Michaelis-Menten relationship. 

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