Renaturation kinetics

ACTIN ASSEMBLY KINETICS MICROTUBULE ASSEMBLY KINETICS PROTEIN POLYMERIZATION KINETICS NUCLEIC ACID RENATURATION KINETICS Nucleic acid structure,... 

Cox, C.S., Phares, C.K. and Schmidt, R.A. (1990) Molecular characterization of the Spirometra mansonoides genome renaturation kinetics, methylation, and hybridization to human cDNA probes. Biochimica et Biophysica Acta 1049, 134-144. 

The presence of ions not only affects canonical base pairs [22], but promotes the formation of triplexes and other non-canonical DNA structures [23]. The effects of these interactions span from modifications of the renaturation kinetics of thermally denaturated DNA [24] to the known anti-tumoral and mutagenic activity of cisplatin [25]. 

The one-gene-one-enzyme concept did imply that the primary structure of the peptide determines the secondary, tertiary, and quaternary structure, and this was established by Anhnsen (1973) by an analysis of the mutant ribonuclease and by the study of chemical modification as well as the denaturation and renaturation kinetics of this enzyme (Anhnsen 1973). 

From Macaya et al. (1978). In this paper the lightest major component of Filipski et al. (1973) had already been resolved into two components. Renaturation kinetics data are from Britten and Smith (1968). 

The renaturation reaction is second order (there are two reacting strands), so one can describe the renaturation kinetics either in terms of the rate constant(s), k2, or in terms of CQt. CQt is the product of the initial concentration (Cq) of denatured DNA (moles of nucleotides per liter) and the time (t) of incubation (seconds) (Britten and Kohne, 1966, 1968). The equation, = 7 + k2 (Cpt) describes the reaction, shows the... 

Intermediate Repetitious DNA. Examination of the renaturation kinetics of salmon sperm DNA shows that at least 80 percent of it consists of repeated sequences (Britten and Kohne, 1968). The fact that the reassociation of these sequences occurs over a large range of C t values suggests that the degree of repetition must vary perhaps between 100 and 100,000. Thermal stability studies of fractions of salmon DNA with different C t values show that there is a broad spectrum in the degree of divergence that has occurred in these families of sequences. 

Hutton, J R. (1977) Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea. Nucleic Acids Res. 4, 3537-3555. 

Special attention has been given to the distribution of unique and repeated sequences along the chromosome and to the construction of polytene chromosomes. Hypotheses on chromomere construction in Drosophila have been based on data of DNA renaturation kinetics (Laird and McCarthy, 1969), formation of rings from DNA fragments (Lee and Thomas, 1973 Thomas et al., 1973), and electron microscopic analysis of repeated sequences (Wu et al., 1972). The models of chromomere construction are presented in Fig. 104 (Laird, 1973). 

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