Protein Function, Enzymes, and Enzyme Kinetics

Protein functions and interactions are infinitely varied in biological species— one of the major problems associated with complete classification of any proteome. Proteins may transport substances myoglobin and hemoglobin (discussed in Chapter 7) transport oxygen, and carbon dioxide, in mammalian blood. Proteins called enzymes catalyze necessary biochemical reactions. The active site of an enzyme contains those amino acids that come in direct contact 

The general theory of enzyme kinetics is based on work of L. Michaelis and M. L. Menten, later extended by G. E. Briggs and J. B. S. Haldane. The basic reactions (E = enzyme, S = substrate, P=product) are shown in equation 2.1  

Assuming that the reactions are reversible and that a one-substrate enzyme-catalyzed reaction is being studied, one can derive the Michaelis-Menten rate  

The Lineweaver-Burk plot uses the reciprocal of the Michaelis-Menten equation in the form of the equation of a straight line, y=mx + h, having the form shown in equation 2.3  

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