Kinase analysis

Clinical Analysis. A wide range of clinically important substances can be detected and quantitated using chemiluminescence or bioluminescence methods. Coupled enzyme assay protocols permit the measurement of kinase, dehydrogenase, and oxidases or the substrates of these enzymes as exemplified by reactions of glucose, creatine phosphate, and bile acid in the following ... 

Kunkel, E. J., Plavec, L, Nguyen, D., Melrose, J., Rosier, E. S., Kao, L. T., Wang, Y., Hytopoulos, E., Bishop, A. C., Bateman, R., et al. (2004). Rapid structure-activity and selectivity analysis of kinase inhibitors by Bio Map analysis in complex human primary cell-based models. ASSAY Drug Dev. Technol. 2 431-441. 

Pelkmans L, Fava E, Grabner H, Hannus M, Habermann B, Krausz E, Zerial M (2005) Genomewide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis. Nature 436 78-86... 

As an example, consider the separation of the creatine kinase isoenzymes, MM, MB, and BB. Mercer has used classical ion-exchange chromatography (DEAE - Sephadex - A50) for the resolution of these three isoenzymes (44) To speed up the separation and ultimately to allow an automated analysis,... 

B3. Baronciani, L., and Beutler, E., Analysis of pyruvate kinase-deficiency mutations that produce nonspherocytic hemolytic anemia. Proc. Natl. Acad. Sci. U.S.A. 90,4324-4327 (1993). 

K6. Kanno, H., Fujii, H., and Miwa, S., Structural analysis of human pyruvate kinase L-gene and identification of the promoter activity in erythroid cells. Biochem. Biophys. Res. Commun. 188, 516-523 (1992). 

K11. Kanno, H Fujii, H., and Miwa, S Molecular heterogeneity of pyruvate kinase deficiency identified by single strand conformational polymorphism (SSCP) analysis. Blood 84 (Suppl. 1), 13a... 

Figure 2 Depiction of the active ( open ) and inactive ( closed ) conformations of Src kinase based on the analysis of x-ray structures of c-Src tyrosine kinase crystallized in its inactive state [7]. The stabilization of the inactive conformation is influenced by multiple events including intramolecular binding of the tyrosine-phosphorylated C-terminus tail to the SH2 domain as well as interactions between the SH3 domain and the SH2-kinase linker. CT, C-terminal NT, N-terminal. 

A murine male germ cell-assocated kinase (mak) was isolated by virtue of its weak homology to the v-ros tyrosine kinase (Matsushime et al., 1990). Although it was isolated because of its homology to a tyrosine kinase, sequence analysis revealed that it belongs to the serine/threonine... 

B-raf, a member of the raf gene family of serine/threonine kinases, is expressed as two major transcripts of 4.0 kb and 2.6 kb in the mouse testis (Wadewitz et al., 1993). B-raf expression is limited to the germ cells and is particularly abundant in early spermatids. Northern hybridization analysis revealed that the two B-ra/transcripts are expressed in a stage-specific manner. Low levels of the 4.0-kb transcript are first... 

---