17-ketogenic steroids

Drug/Lab test interactions Naproxen use may result in increased urinary values for 17-ketogenic steroids. Temporarily discontinue naproxen therapy 72 hours before adrenal function tests are performed. 

High-dosage penicillin produces abnormally high concentrations of 17-ketogenic steroids in the blood and high concentrations of 17-ketosteroid in the urine (257). 

Sobel C, Golub OJ, Henry RJ, Jacobs SL, Basu GK. Study of the Norymberski methods for determination of 17-ketogenic steroids (17-hydroxycorticosteroids) in urine. J Clin Endocrinol Metab 1958 18 208-21. 

Some estimates of the precision of various typical modem procedures are given in Table 3. General clinical chemists may well be surprised at some of the values when compared with objective experience in other areas. Thus it is well known that the estimation of serum bilimbin has a coefficient of variation (C.V.) of around 15% in most laboratories, and that with creatinine a value of 8-10% is fairly normal (W3). In a recent blind (but not double-blind) interlaboratory trial of a standardized method for 17-ketogenic steroids and one for 17-ketosteroids, Gray et al. (GIO) obtained C.V. s varying between 4% and 14% for both methods. Six of the ten laboratories cooperating in the trial had special steroid experience and were asked to obtain duplicate estimates of any thirty routine specimens of urine. These results are for two well-established and relatively simple procedures. 

This statement too may cause raised eyebrows but an objective assessment of errors and costs can support it. Thus, De Courcy s trichloroacetic acid reagent (D2) is capable of convenient application to the detection of pregnanetriol in the nonketonic fraction from one-sixteenth of a 24-hour collection from men (100-150 ml) on simple LLC paper chromatograms (a TLC method could no doubt also be devised for this). As she pointed out, the same quantity can be obtained from one-thousandth of a 24-hour collection (1.5-2 ml) of urine from some patients with adrenal hyperplasia. Quantitative estimation by the same reaction after elution from the paper or TLC chromatogram could also be used either by one of the many variations of the micro-17-ketogenic steroid methods or by the same fluorescence reaction (e.g., W7). Further confirmation of identity could be obtained by using a small fraction of the latter after the periodate oxidation for a paper LLC, or TLC, chromatogram and application of the Zimmerman reaction to demonstrate the formation of etiocholanolone (B32). 

V7. Vestergaard, P., and Sayegh, J. F., Semi-automated assays for urinary 17-ketogenic steroids a comparison of bismuthate and periodate oxidation methods. Clin. Chim. Acta 14, 247-262 (1966). 

The metyrapone test may be used diagnostically to evaluate the proper functioning of the anterior pituitary gland. When administered orally, metyrapone inhibits the activity of 11-beta-hydroxylase, which is necessary for the synthesis of cortisol, corticosterone, and aldosterone, promotes the release of corticotropin, which in turn increases production of the precursors (11-deoxycortisol and 11-deoxycorticosterone), and enhances the appearance of 17-hydroxycorticoster-oids and 17-ketogenic steroids. 

Naproxen may interfere with some tests for 17-ketogenic steroids (5). 

Simultaneous Determination of Urinary Estrogens, Individual ll-Deoxy-17-ketosteroids, Total 17-Keto Steroids, Total 17-Ketogenic Steroids, Pregnanediol, and Plasma Cortisol During the Menstrual Cycle and in Early Pregnancy... 

A Simple Method for the Determination of Urinary 17-Ketogenic Steroid Fractions by Differential Solvent Extraction. I. Study of the Feasibility of Solvent Fractionation by Gas Chromatography Horumon to Rinsho 27(8) 903-908 (1979) CA 92 2788m... 

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