Trichloroacetic acid reagent

For their detection, the papers may be sprayed with reagents commonly used for the detection of keto and aminodeoxy sugars. Those of particular value include the direct Ehrlich reaction, the orcinol-trichloroacetic acid reagent, the chlorine-benzidine reagent for the detection of —NH—CO— groupings, and the Bial reaction as modified by Bohm und Baumeister. The limit of detection with the orcinol-trichloroacetic acid reagent is reported to be 5 ixg. of V-acetylneuraminic acid. For the detection of methoxyneuraminic acid, ninhydrin may also be used. 

This statement too may cause raised eyebrows but an objective assessment of errors and costs can support it. Thus, De Courcy s trichloroacetic acid reagent (D2) is capable of convenient application to the detection of pregnanetriol in the nonketonic fraction from one-sixteenth of a 24-hour collection from men (100-150 ml) on simple LLC paper chromatograms (a TLC method could no doubt also be devised for this). As she pointed out, the same quantity can be obtained from one-thousandth of a 24-hour collection (1.5-2 ml) of urine from some patients with adrenal hyperplasia. Quantitative estimation by the same reaction after elution from the paper or TLC chromatogram could also be used either by one of the many variations of the micro-17-ketogenic steroid methods or by the same fluorescence reaction (e.g., W7). Further confirmation of identity could be obtained by using a small fraction of the latter after the periodate oxidation for a paper LLC, or TLC, chromatogram and application of the Zimmerman reaction to demonstrate the formation of etiocholanolone (B32). 

N,N-Dimethylaniline Isopropenyl acetate reagent, albumin 2-Amino-4,6-dinitrophenol reagent, albumin detection Trichloroacetic acid reagent, albumin testing Zinc acetate... 

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