Jelinski

An elegant NMR experiment by the group of Lynn Jelinski at Cornell University has established that at least part of the microcrystals is built up from the polyalanine repeats in the protein chains. These experiments, which were made on C-enriched proteins produced by feeding the spiders C-labeled alanine, showed that there were two populations of alanine side chains, one ordered and oriented perpendicular to the fiber axis and a second less ordered. Jelinski s interpretation is that parts of the polyalanine sequences are incorporated as p strands in the microcrystals with an orientation parallel to the fiber axis. Whether or not the Gly-Gly-X repeats also form P strands in the microcrystals remains an open question. 

Simmons, A.H., Michal, C.A., Jelinski, L.W. Molecular orientation and two-component nature of the crystalline fraction of spider dragline silk. Science 271 84-87, 1996. 

Jelinsky SA et al. Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci USA 1999 96 1486-1491. 

Clark, P., in Nanofabrication and Biosystems Integrating Materials Science, Engineering and Biology (H. C. Hoch, L. W. Jelinski, and H. G. Craighead, Eds.), p. 357. Cambridge University Press, Cambridge, UK (1996). 

Jelinski, L. Overview biological, carbon and theory issues, in Seigel, R. W., Hu, E. and Roco, M. C. (eds), R D Status and Trends in Nanoparticles, Nanostructured Materials and Nanodevices in the United States, International Technology Research Institute, Baltimore, MD, USA, 1997, pp. 161-169. 

We wish to thank Dr. Y. Y. Lin for assistance with the GC-MS experiments. We are also indebted to Drs. L. W. Jelinski, J. B. Wooten, and G. Odian for helpful discussions during the course of this work. Partial support for this research was provided by grants (to R.E.S.) from the National Science Foundation (DMR-8617595), the Professional Staff Congress-City University of New York, and the College of Staten Island. 

A. G. Ludwick, L. W. Jelinski, D. Live, A. Kintanar and J. J. Dumais, Association of peptide chains during merrifield solid-phase peptide synthesis, a deuterium NMR study, J. Am. Chem. Soc., 1986, 108, 6493-6496. 

Kozack R, Seo KY, Jelinsky SA, et al. Toward an understanding of the role of DNA adduct conformation in defining mutagenic mechanism based on studies of the major adduct (formed at N2-dG) of the potent environmental carcinogen benzo[a]pyrene. Mutat Res 2000 450(1-2) 41-59. 

Bovey, F A, Jelinski, L, Mrrau, P A Nuclear Magnetic Resonance Spectroscopy, 2nd ed, Academic Press New York, 1988... 

Batchelder, L. S., Sullivan, C. E., Jelinski, L. W., and Torchia, D. A. (1982). Characterization of leucine side-chain reorientation in collagen fibrils by solid state 2H NMR. Proc. Natl. Acad. Sci. 79, 386—389. 

MJL81] Martin C, Jelinsky P, Lampton M and Malina RF 1981 Rev. Sci. Instrum. 52 1067. [Moo70] Moore ChE 1970 Ionization potentials and ionization limits derived from the analyses of optical spectra, US Govt. Printing Office, Washington DC NSRDS-NBS34. 

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