Isolation of Lipoproteins from Human Plasma

Lipoproteins transport lipids in blood an early classification system was established using centrifugation methods, which are based on their different densities. Lipoproteins are classified as chylomicrons, largest and lowest in density 

Standard centrifugation procedures used for fractionation of lipoproteins in human blood plasma are made by sequential flotation using KBr, NaBr, NaCl, or a combination of these salts to produce a solution with determined density, in which a selected lipoprotein fraction moves upward. 

Using self-formed iodixanol gradients, a novel rapid method for the separation of plasma lipoprotein has recently been described.19 The centrifugation time is reduced to 4 h, but the gradient must be collected carefully, in 0.1-0.2-mL fractions. 

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