Isolation and physicochemical properties

Kanno, C., Shimizu, M. and Yamauchi, K. 1975. Isolation and physicochemical properties of a soluble glycoprotein fraction of milk fat globule membrane. Agr. Biol. Chem. 39, 1835-1842. 

Gebre-Mariam, T. Schmidt, P.C. Isolation and physicochemical properties of enset starch. Starch/Starke 1996, 48, 208-214. 

Chan, K.F.J. Graves, D.J. Isolation and physicochemical properties of active complexes of rabbit muscle phosphorylase kinase. J. Biol. Chem., 257, 5939-5947 (1982)... 

Oki T, Kitamura I, Yoshimoto A, Matsuzawa Y, Shibamoto N, Ogasawara T, Inui T, Takamatsu A, Takeuchi T, Masuda T, Hamada M, Suda H, Ishizuka M, Sawa T, Umezawa H. Antitumor anthracycline antibiotics, acladnomycin A and analogues. 1. Taxonomy, production, isolation, and physicochemical properties. J Antibiot 1979 32 791-8(X ). 

Omura, S, Iwai, Y, Nakagawa, A, Iwata, R, Takahashi, Y, Shimizu, H, Tanaka, H, Thiotetromycin, a new antibiotic. Taxonomy, production, isolation, and physicochemical and biological properties, J. Antibiot., 36, 109-114, 1983. 

Thanh, V.H. K. Shibasaki. P-Conglycinin from soybean proteins Isolation and immunological and physicochemical properties of the monomeric forms. Biochim. Biophys. Acta 1977, 490, 370—384. 

Serum albumin, the most abundant plasma protein, has been subjected to extensive studies which resulted in a wealth of information on its chemical and physicochemical properties. Only recently has the amino acid sequence of bovine and human serum albumins become known. Prior to the availability of the amino acid sequence, immunochemical studies on BSA or HSA were hampered by the inability to assign an immunochemically reactive fragment within the sequence of the albumin molecule. With the availability of the sequence, it is becoming possible to identify immunochemically reactive fragments of known sequences. Thus fragments 11-193 and 377-571 were isolated from BSA. These fragments were shown to have rather unusual immunochemical properties. Each... 

Cherian et al. [119] also extracted cellulose nanofibres from pineapple leaf fibres using acid-coupled steam treatment. The strucmral and physicochemical properties of the pineapple leaf fibres were studied by environmental scanning electron microscopy (ESEM), AFM and TEM and X-ray diffi action (XRD) techniques. The acid-coupled steam explosion process resulted in the isolation of PALF nanofibres having a diameter range of 5-60 nm. Figure 1.24a and b shows the AFM and TEM images of nano fibres obtained from pineapple leaf fibres. AFM and TEM support the evidence for the isolation of individual nanofibres from PALF. 

The distribution, isolation, determination, physicochemical properties, structure, biosynthesis, metabolism, etc. of immunoglobulin A have been reviewed. The use of affinity labelling to explore the topography of the antibody-combining sites of immunoglobulins and the specificities of antibodies have also been reviewed. 

In all four flavanones were identified as components of the fractions examined. (S)-(-)-Pinostrobin (16, 17) (5-hydroxy-7--methoxyflavanone, 17) was isolated from all of the three fractions examined and was identified from its spectral characteristics and physicochemical properties (Fig. 3). The identification of flavanones by mass spectrometry (MS) is a relatively simple procedure. The major fragmentations of flavanones such as pinostrobin (Fig. 4) yield ions which are characteristic for the A and B rings of the molecule indicating the level of substitution on these rings (e.g. ions at m/e 166 and 104 respectively for pinostrobin) (18). 

Biotechnology creates value upstream in a production process for chemicals, intermediates, and pharmaceutical ingredients. This created value must be conserved in the following isolation and purification or downstream process. Innovation is needed both in the upstream process (USP) and in the downstream process (DSP) to minimize losses, remove side products, and still be environmentally tenable. Bioieactions are mostly carried out in aqueous solutions and product isolahon can be problematic because of the similarity of solubility and physicochemical properties of the product, substrate, and side product. However, effective methods exist to address most isolation and purification problems. Alternative DSP practices are increasingly used and... 

Spiro, R. G., 1960, Studies on fetuin, a glycoprotein of fetal serum I. Isolation, chemical composition, and physicochemical properties, J. Biol. Chem. 235 2860-2869. 

Sliwkowski MX, StadtmanTC. 1988. Selenium-dependent glycine reductase differences in physicochemical properties and biological activities of selenoprotein A components isolated from Clostridium sticklandii and Clostridium purinolyticum. Biofactors 1 293-6. 

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