Glycine reductase

Clostridal glycine reductase is the bacterial enzyme which has been investigated most thoroughly . This enzyme catalyzes the reductive deamination of glycine to ammonia and acetate with the concomitant synthesis of ATP (Eq. 2). As has been 

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Arkowitz RA, RH Abeles (1989) Identification of acetyl phosphate as the product of clostridial glycine reductase evidence for an acyl enzyme intermediate. Biochemistry 28 4639-4644. 

Glycine reductase selenoprotein A Energy metabolism Clostridium sticklandii 5, 99, 100... 

Glycine reductase selenoprotein B Energy metabolism Eubacterium acidaminophilum 101-103... 

Glycine reductase protein A C. sticklandii Turner and Stadtman (1973)... 

Figure 12.1. Requirement for selenium in glycine reductase activity in C. sticklandii when cultured in a defined medium. Data from Turner and Stadtman (1973).

Cone JE, del Rio RM, Davis JN, Stadtman TC. 1976. Chemical characterization of the selenoprotein component of clostridial glycine reductase identification of selenocysteine as the organoselenium moiety. Proc Natl Acad Sci USA 73 2659-63. 

Hermann K, Andreesen JR. 1989. Reductive cleavage of sarcosine and betaine by Eubacterium acidaminophilum via enzyme systems different from glycine reductase. Arch Microbiol 153 50-9. 

Kreimer S, Andreesen JR. 1995. Glycine reductase of Clostridium litorale. Cloning, sequencing, and molecular analysis of the grdAB operon that contains two in-frame TGA codons for selenium incorporation. Eur J Biochem 234 192-9. 

Meyer M, Granderath K, Andreesen JR. 1995. Purification and characterization of protein PB of betaine reductase and its relationship to the corresponding proteins glycine reductase and sarcosine reductase from Eubacterium acidaminophilum. Eur J Biochem 234 184-91. 

Sliwkowski MX, StadtmanTC. 1988. Selenium-dependent glycine reductase differences in physicochemical properties and biological activities of selenoprotein A components isolated from Clostridium sticklandii and Clostridium purinolyticum. Biofactors 1 293-6. 

StadtmanTC. 1989. Clostridial glycine reductase protein C, the acetyl group acceptor, catalyzes the arsenate-dependent decomposition of acetyl phosphate. Proc Natl Acad Sci USA 86 7853-6. 

StadtmanTC, Davis JN. 1991. Glycine reductase protein C. Properties and characterization of its role in the reductive cleavage of Se-carboxymethyl-selenoprotein A. J Biol Chem 266 22147-53. 

Tanaka H, Stadtman TC. 1979. Selenium-dependent clostridial glycine reductase. Purification and characterization of the two membrane-associated protein components. J Biol Chem 254 447-52. 

Turner DC, Stadtman TC. 1973. Purification of protein components of the clostridial glycine reductase system and characterization of protein A as a selenoprotein. Arch Biochem Biophys 154 366-81. 

Wagner M, Sonntag D, Grimm R, et al. 1999. Substrate-specific selenoprotein B of glycine reductase from Eubacterium acidaminophilum. Biochemical and molecular analysis. Eur J Biochem 260 38-49. 

GLYCINE REDUCTASE GLYCINE SYNTHASE Glycogen debranching enzyme, DEBRANCHING ENZYME Glycogenolysis tracer kinetics,... 

This enzyme also contains an N-terminal pyruvoyl residue as does one subunit of a selenium-containing glycine reductase which utilizes a dithiol to convert glycine into acetate with coupled formation of ATP ... 

Glycine reductase is a complex enzyme530 564 566 that catalyzes the reductive cleavage of glycine to acetyl phosphate and ammonia (Eq. 15-61) with the... 

Glycine N-methyltransferase 591,593s Glycine reductase 824 Glycocalyx 6... 

Glycine can be reduced to acetate and ammonia by the selenium-dependent clostridial glycine reductase system (Eq. 15-61). A variety of additional products can be formed from glycine as is indicated in Fig. 24-20. Hippuiic acid (Box 10-A), the usual urinary excretion product in the "detoxication" of benzoic acid, is formed via benzoyl-CoA (Eq. 24-43) ... 

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