Blocked interfaces

FIGURE 20 A way to prevent frequent system failure caused by punching of the electrode in the vial closure septum due to sticking of vials at the capillary interface block of the most frequently used QC performance Beckman MDQ CE system is the use of sandwich racks. The cover of these racks avoids vials coming out of the racks. 

The ProteomeLab PA800 instrument was set up to analyze a sequence of five different labeled rMAb samples with a total of 10 duplicate injections. On many occasions, the electrophoretic analysis of approximately 60% of the injections did not take place due to pressure failures in the instrument. Upon closer inspection, some of red rubber caps of the gel matrix-containing vials were missing, electrodes were bent, and capillaries were broken. Additionally, it was observed that the interface block was coated with a sticky film of gel matrix. It was apparent that the build-up of gel buffer was causing the red rubber caps to remain stuck to the interface block and interfere with the contact of vials in subsequent steps. To improve the removal of gel buffer, the interface block and the surrounding areas were carefully flushed with deionized water. Then, cotton swabs were used to wipe the grooves on... 

The readings from the pickups are processed by the interface block Agilent. The investigation program is given by a computer. Scanning and data processing are performed automatically. 

It is believed that it is connected with decrease of size of interface blocks. The obtained films are difficult doping in a gas phase due to its high density. Conductivity of initial films reaches 10-200 (Ohm-cm ) when doped with bromine or iodine in a solution [51]. 

In addition to the substrate specificity profiling of KSl, the effect of salt concentration upon the oligomeric state of the KS was examined. BaeJ KSl was found to dimerise under low salt conditions (5 mM), albeit in an equilibrium with the monomer, and in the presence of high salt (>250 mM) was found to be exclusively monomeric. The acylation of KSl was found to be 3 times more effective when in the monomeric state. With knowledge from crystal structures that the dimer interface blocks part of the KS binding pocket, it is conceivable that relying on diffusion to promote substrates into the KS active site is relatively artificial compared to the native arrangement of a tethered acyl-ACP. 

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