Integrated optical SPR sensor

Sheridan AK, Harris RD, Bartlett PN, Wilkinson JS (2004) Phase interrogation of an integrated optical SPR sensor. Sensors Actuators B Chem 97 114-121... 

Since the demonstration of the first integrated optical SPR sensor by researchers at the University of Twente in the late 1980s [66], integrated optical SPR sensors using slab waveguides [67] and channel waveguides [68] have been developed. 

Fig. 22 Integrated optical SPR sensor with wavelength modulation [70]...

Over the last 15 years, numerous SPR sensors based on optical fibers or integrated optical waveguides have been proposed. Waveguide-based SPR sensors use either wavelength or intensity modulation and offer compact and miniature sensing elements with the ability to perform localized measurements in hard-to-access locations. 

In recent years, first prototypes of SPR biosensor systems integrating these elements have been reported and their application for detection of biological analytes in the field have been demonstrated. Undoubtedly, advances in the development of the key elements - sample preparation technology, microfluidics, biomolecular recognition elements, SPR optical platform - will further stimulate this effort and eventually lead to in-field SPR sensor systems becoming a commercial reality. 

Mouvet et al. developed an integrated optical (10) SPR sensor for simazine [23]. A triazine derivative was immobilized on the sensor using the... 

Fluorescence measurements and detection can either be made under steady-state or time-resolved conditions. Some of the commonly used measurement techniques focus on changes in optical properties such as fluorescence intensity quenching, phase fluorometry (lifetime), polarization, surface plasmon resonance (SPR), and evanescent waves. Here, we will present detector systems based for (a) fluorescence intensity quenching and (b) phase fluorometry in detail. A few example references of integrated optical sensor systems based on the various optical measurement techniques are given in Table 1 and the reader is encourage to review those papers if more details are desired. 

The first commercial SPR was launched hy Pharmacia Biosensor AB (presently Swedish BIAcore AB) in 1990. Since then, the device has been refined and now BIAcore [37] offers several models (BIACORE 3000, BIACORE 2000, BIACORE 1000, BIACORE X, J, Q, S51, and C models). The biosensors of BIACORE 1000 to 3000 are fully automated instruments, with a disposable sensor chip, an optical detection unit, an integrated micro-fiuidic cartridge, an autosampler, method programming and control software. Less expensive manually controlled alternatives cU e the BIACORE x and BiacoreQuant . 

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