Instrumentation, chemiluminescent

Chemiluminescent labeling systems have been developed, based on the incorporation of fluorescein-11-dUTP into a DNA probe. An anti-fluorescein antibody covalently bound to the enzyme HRP is then bound to the incorporated fluorescein label. HRP catalyses the breakdown of luminol and the chemiluminescent signal is detected by autoradiography with X-ray film or by fluorescence scanning instrumentation. Chemiluminescence is more sensitive than enzyme-based color detection systems. Furthermore the labeled gene probes are stable and give results quickly (160). 

See also Atomic Absorption Spectrometry Principles and Instrumentation. Chemiluminescence Overview. Chromatography Overview. Flow Injection Analysis Principles Instrumentation. Ion-Selective Electrodes Overview. Quality Assurance Quality Control Reference Materials. Sample Handling Sample Preservation. Sampling Theory. Water Analysis Overview Organic Compounds. Water Determination. 

K. Van Dyke, ed.. Bioluminescence and Chemiluminescence Instruments and Applications, CRC Press, Boca Raton, Fla., 1985. 

An instrument for measuring nitrogen oxides based on chemiluminescence is shown in Fig. 13.49. The ozone required for the reaction is produced in the ozone generator, which is part of rhe device. One of the reaction chamber walls is an optical filter through which a red-sensitive photomultiplier tube measures the chemiluminescence radiation intensity and converts it into a current signal. 

Stanley, P. E. (1992). A survey of more than 90 commercially available luminometers and imaging devices for low-light measurements of chemiluminescence and bioluminescence, including instruments for manual, automatic and specialized operation, for HPLC, LC, GLC and microtiter plates. Part I descriptions. T. Biolumin. Chemilumin. 7 77-108. 

Instrumental methods of peroxide analysis feature polarography, which is used to detn hydroperoxides, peroxyesters and diacyl peroxides as well as dicyclohexyl peroxydicarbonate in polystyrene. Other techniques include infrared (800 to 900cm 1) chemiluminescent analysis for kinetic studies, and chromatography for the identification and separation of peroxides in complex mixts (Refs 5,6, 7,14,15,16,17, 20 21)... 

Nitric oxide (NO) and nitrogen dioxide (NO2) were monitored by a Bendix chemiluminescence instrument. However, since the "NO2" readings of this type of instrument are known to include the contribution of HNO3, HONO, PAN, and other organic nitrates ( ), we report these readings as "gaseous nitrate."... 

Supercritical fluid chromatography (SEC) was first reported in 1962, and applications of the technique rapidly increased following the introduction of commercially available instrumentation in the early 1980s due to the ability to determine thermally labile compounds using detection systems more commonly employed with GC. However, few applications of SEC have been published with regard to the determination of triazines. Recently, a chemiluminescence nitrogen detector was used with packed-column SEC and a methanol-modified CO2 mobile phase for the determination of atrazine, simazine, and propazine. Pressure and mobile phase gradients were used to demonstrate the efficacy of fhe fechnique. 

Figure 25 Comparison of the molar mass distribution from original sample of pullulan (1) and that heated by the rate 2.5°C/min from 40°C to 220°C (2) in oxygen directly in chemiluminescence instrument. The line 3 is the same experiment with sample impregnated with MMMC.

Sievers Instruments. Gas-phase chemiluminescence detector http / / www.SieverInst.com/... 

The last three decades have seen a slow but constant evolution in the conception of instruments for chemiluminescence (CL) or bioluminescence (BL). Initially... 

SP Colowick, NO Kaplan. In MA DeLuca, ed. Methods Enzymol, vol LVII. Bioluminescence and Chemiluminescence, section IX. Instrumentation and Methods. New York Academic Press, 1978, pp 529-559. 

This review deals mainly with BL analytical applications in the last 10-15 past years, but some previous fundamental works are also listed. In Table 3 some fundamentals references of general interest and the findings of recent symposia on this topic are collected. In the journal Luminescence, the Journal of Biological and Chemical Luminescence (previously Journal of Bioluminescence and Chemiluminescence) are also reported surveys of the recent literature on selected topics (like ATP or GFP applications), instruments, and kits commercially available. 

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