Electrogenerated chemiluminescence instrumentation

The distance (and hence volume) between the confluence point of the sample and reagent streams and the detection cell needs to be optimized for the kinetics of the reaction used. The flow cell must be transparent to the wavelength of the chemiluminescence emission and inert to the chemical reaction or solvent system glass, quartz, and Teflon tubing are commonly employed. Instrumentation for electrogenerated chemiluminescence requires suitable electrodes and a potentiostat to facilitate and control the electrochemistry. Gold, platinum, or carbon working electrodes are placed in the observation cell with counter and reference electrodes situated downstream. 

Fig. 5.18 Schematic diagram of the flow injection manifold and instrumentation used for the electrogeneration of chemiluminescence. Reprinted with permission from Ref. [109]. Copyright 1995 The Royal Society of Chemistry...

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