Insertion sequence

F. Martinez-Abarca, S. Zekri, and N. Toro, Characterization and in vivo of a Sinorhi-zobium meliloti group II intron associated with particular insertion sequences of the IS630-Tcl/IS3 retroposon superfamily. Mol. Microbiol. 28 1295-1306 (1998). 

Masui, S., Kamoda, S., Sasaki, T. and Ishikawa, H. (1999) The first detection of the insertion sequence ISW1 in the intracellular reproductive parasite Wolbachia. Plasmid 42, 13-19. 

The intention of this section is to discuss some aspects of the chemistry of metal complexes in solution which are related to the conversion of carbon monoxide and hydrogen to organic products. In order to provide a framework for the initial part of the discussion, we have adopted the simple reduction/insertion sequence shown in Eq. (2). 

Scheme 8.36. Formation of a bicycle by an insertion-carbometallation-insertion sequence.

Mycobacteria of the Mycobacterium avium complex are implicated in disseminated bacterial infections in AIDS patients. RFLP studies followed by hybridization with radiolabeled probe specific for an insertion sequence in M. avium (IS 1311) have been useful for typing M. avium stains (R2). A variety of molecular techniques are available for the diagnosis of Chlamydia trachomatis infection. In addition to PCR, a method based on the ligase chain reaction has also been found to be sensitive to the detection of C. trachomatis infection in urine specimens collected from male and female subjects (VI). The differentiation between low-risk genotypes of human papilloma virus (HPV 6 or 11) from genotypes of high... 

McDougall S, Neilands JB (1984) Plasmid- and Chromosome-coded Aerobactin Synthesis in Enteric Bacteria Insertion Sequences Flank Operon in Plasmid-mediated Systems. J Bacteriol 159 300... 

Amplify the insert sequence using suitable oligonucleotide primers. 

The In-Fusion method is both insert sequence-independent and enables cloning of PCR products directly into any cloning or expression vector (Fig. 2.Tc). The mechanism of the reaction has not been fully reported but relies on the presence of... 

Amplify the desired insert sequence using appropriately designed PCR primers. The 5 -end of the primers must incorporate the following sequences ... 

Kroon, J. et al.. Cloning and structural analysis of the anthocyanin pigmentation locus Rt of Petunia hybrida. characterization of insertion sequences in two mutant alleles. Plant J., 5, 69, 1994. 

Kulkosky J, Jones KS, Katz RA, Mack JPG, Skalka AM. Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transposases. Mol Cell Biol 1992 12 2331-2338. 

Ellis JE, Yarlett N, Cole D, Humphreys MJ, Lloyd D (1994) Antioxidant defenses in the microaerophilic protozoan Trichomonas vaginalis—comparison of metronidazole-resistant and sensitive strains. Microbiol-SGM 140 2489-2494 Haggoud A, Reysset G, Azeddoug H, Sebald M (1994) Nucleotide sequence analysis of two 5-nitroimidazole resistance determinants from Bacterioides strains and of a new insertion sequence upstream of the two genes. Antimicrob Agents Chemother 38 1047-1051... 

Bacteria have two classes of transposons. Insertion sequences (simple transposons) contain only the sequences required for transposition and the genes for proteins (transposases) that promote the process. Complex transposons contain one or more genes in addition to those needed for transposition. These extra genes might, for example, confer resistance to antibiotics and thus enhance the survival chances of the host cell. The spread of antibiotic-resistance elements among disease-causing bacterial populations that is rendering some antibiotics ineffectual (pp. 925-926) is mediated in part by transposition. 

Nakatsu, C., Ng, J-, Singh, R., Straus, N. Wyndham, C. (1991)- Chlorobenzoate catabolic transposon Tn5271 is a composite class I element with flanking class II insertion sequences. Proceedings of the National Academy of Sciences USA, 88, 8312-16. 

A four-base overlap between dihydrofolate reductase and thymidylate synthase has been found201 in the DNA of phage T4. A transposable DNA insertion sequence (see Section D,5) in E. coli encodes two genes, one of which is contained within the other and which is transcribed from the opposite strand of DNA.202 The double-stranded RNA of a reovirus produces two peptides from the same sequence using two different AUG initiation codons in different reading frames 203... 

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