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Insect cells, luciferase

Viviani, V. R. (2002). The origin, diversity, and structure function relationships of insect luciferases. Cell. Mol. Life Sci. 59 1833-1850. [Pg.447]

The successful transfer of recombinant DNA into plants was vividly illustrated by an experiment in which the luciferase gene from fireflies was introduced into the cells of a tobacco plant (Fig. 9-29)—a favorite plant for transformation experiments because its cells are particularly easy to transform with Agrobacterium. The potential of this technology is not limited to the production of glow-in-the-dark plants, of course. The same approach has been used to produce crop plants that are resistant to herbicides, plant viruses, and insect pests (Fig. 9-30). Potential benefits include increased yields and less need for environmentally harmful agricultural chemicals. [Pg.332]

HEK-293 hGH High-Five hsp70 HSV IPTG IRES kb Lac LCR LoxP LUC MCS human embryonic kidney cells human growth hormone TM BTI-TN-5B1-4 (cell line derived from the insect Trichoplusia ni) heat shock protein 70 herpes simplex virus isopropyl 1 -thio-fi-D-galactopyranoside internal ribosomal entry site kilobases lactose operon/repressor locus control region locus of crossover of PI luciferase isolated from firefly multiple cloning site... [Pg.536]

Two types of ludferase genes, cloned from bacteria and firefly, are used as sensitive reporter systems in a wide variety of cells such as barterial, yeast, insect, animal, and plant cells. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly ludferase is a single polypeptide spedfied by the luc gene. The two types of luciferase catalyze... [Pg.628]

Both firefly luciferase (see below) and bacterial luciferase are sensitive reporters of gene expression suitable for the study of regulatory sequences (promoter, enhancer, and terminator) of cellular differentiation and morphogenesis, and of responses to environmental and developmental changes (53,54). The two reporter systems have been used in bacteria, yeast, fungi, insects, plants, and mammalian cells. As far as in vitro assays are concerned, both reporter systems may be considered equally useful. For in vivo applications, however, one system may turn out to be more suitable than the other due to the strengths and weaknesses inherent to each reporter system. [Pg.638]


See other pages where Insect cells, luciferase is mentioned: [Pg.176]    [Pg.99]    [Pg.1164]    [Pg.91]    [Pg.86]    [Pg.102]    [Pg.177]    [Pg.627]    [Pg.639]   
See also in sourсe #XX -- [ Pg.80 ]




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Luciferases

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